青花菜BoBURP1基因的克隆与表达分析

BURP是一类存在于植物中的特有蛋白,在生长发育和逆境响应等方面起重要作用。试验从青花菜中克隆到一个BURP基因,命名为BoBURP1,在生物信息学分析的基础上,用RT-PCR研究了它在不同器官中的表达模式。研究结果表明,BoBURP1的基因组DNA全长2 030 bp,具2个内含子;编码区全长为1 872 bp,编码623个氨基酸;推导的蛋白质分子量为70.795 6 ku,等电点为8.65。系统发育分析结果表明,BoBURP1与12种不同植物的同源蛋白可分为3组,BoBURP1与十字花科植物同源序列的相似性最高,在进化树上聚为一组。RT-PCR结果表明,BoBURP1在根、茎、叶、花蕾、花和角果中均有表达,叶、花蕾和花的表达量最高,而根和茎中的表达量最低。

Cloning and expression analysis of BoBURP1 gene from Brassica oleracea var. italica

BURPs are plant-specific proteins, which play important roles in growth, development as well as stress responses. In this study, a BURP gene, designated as BoBURP1, was isolated from broccoli. Bioinformatics analysis has been performed, and expression patterns in different organs were then conducted by means of RT-PCR. Results indicated that the genomic DNA of BoBURP1 was 2 030 bp in length with 2 introns, and the full length coding sequence was 1 872 bp in size encoding 623 amino acids. The molecular weight of the deduced protein was 70.7956 ku with an isoelectric point of 8.65. Phylogenetic analysis results revealed that BoBURP1 and its 12 homologous sequences could be divided into three groups, and the highest identities existed among Cruciferae plants which clustered in the same group. RT-PCR results showed BoBURP1 gene expressed in roots, stems, leaves, flower buds, flowers and young siliques, and the highest was observed in leaves, flower buds and flowers, while the lowest were found in both roots and stems.