| 从许氏平鲉体表分离海分枝杆菌的鉴定及其脂多糖的抗原性分析 |
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| 引用本文： | 绳秀珍,孟鹏,徐晓丽,战文斌.从许氏平鲉体表分离海分枝杆菌的鉴定及其脂多糖的抗原性分析[J].水产学报,2011,35(10):1524-1531. |
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| 作者姓名： | 绳秀珍 孟鹏 徐晓丽 战文斌 |
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| 作者单位： | 中国海洋大学海水养殖教育部重点实验室,山东青岛,266003 |
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| 基金项目： | 国家自然科学基金项目(31072232,31172429) |
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| 摘 要： | 从许氏平鲉粘液、背鳍、鳃等部位分离筛选到同一菌株FZ09,经形态学观察、生理生化特性及热激蛋白65(HSP65)基因序列分析,鉴定菌株FZ09为海分枝杆菌。分别用酚-氯仿-甲醇法、乙醇-酚水法和传统热酚水法提取菌株FZ09的脂多糖(LPS),分析了LPS的组成和抗原性差异。SDS-PAGE结果表明,3种方法提取的LPS条带分布基本相同,主要条带分子量分别为14、16和23 ku,其中以乙醇-酚水法提取的LPS含量和纯度最高,热酚水法提取的LPS条带最多,抗原性最好。Western-blotting显示,海分枝杆菌抗血清主要与LPS的14 ku条带发生特异性免疫反应。高碘酸氧化免疫印迹结果表明,仅传统热酚水法提取的LPS在14 ku分子量区域有弱的阳性条带出现,其他方法提取LPS与抗血清的免疫反应呈阴性。高碘酸氧化ELISA反应显示,LPS经高碘酸氧化后与海分枝杆菌FZ09抗血清的反应活性降低,OD405明显减弱。
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| 关 键 词： | 许氏平鲉 海分枝杆菌 脂多糖 抗原性 |
| 收稿时间： | 4/11/2011 8:33:50 PM |
| 修稿时间： | 7/31/2011 1:15:33 PM |
Identification and lipopolysaccharide antigenicity analysis of Mycobacterium marinum isolated from Sebastes schlegeli |
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| SHENG Xiu-zhen,MENG Peng,XU Xiao-li and ZHAN Wen-bin.Identification and lipopolysaccharide antigenicity analysis of Mycobacterium marinum isolated from Sebastes schlegeli[J].Journal of Fisheries of China,2011,35(10):1524-1531. |
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| Authors: | SHENG Xiu-zhen MENG Peng XU Xiao-li and ZHAN Wen-bin |
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| Institution: | SHENG Xiu-zhen,MENG Peng,XU Xiao-li,ZHAN Wen-bin(Laboratory of Pathology and Immunology of Aquatic Animals,Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao 266003,China) |
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| Abstract: | Mycobacterium marinum is an opportunistic pathogen which can infect 150 kinds of aquatic animals.In this paper,the same bacteria strain FZ09 was isolated from the mucus,dorsal fins and gills of Sebastods schlegeli,and was identified as Mycobacterium marinum by physiological,biochemical characterization and heat shock protein 65 DNA sequence analysis.Lipopolysaccharide(LPS)was extracted from strain FZ09 by phenol-chloroform-methanol method,ethanol-phenol-water method and hot phenol-water method,respectively,... |
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| Keywords: | Sebastes schlegeli Mycobacterium marinum lipopolysaccharide antigenicity |
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