固相萃取-高效液相色谱法测定乳制品中三氯蔗糖

建立乳制品中三氯蔗糖的固相萃取高效液相色谱检测方法.采用0.2％乙酸水溶液沉淀蛋白,提取三氯蔗糖,然后通过C18固相萃取柱进行净化浓缩后,采用高效液相色谱-蒸发光散射检测仪进行检测,该方法0.1～0.8g/L范围内以三氯蔗糖进样质量的对数和相应峰面积的对数做标准曲线,R2为0.9991,相关性良好,加标回收率在94％～102％之间,检出限为2.0mg/L(RSN=3).

Determination of Sucralose in Dairy Foods by SPE-HPLC

A high performance liquid chromatographic method with evaporative light scattering detection (HPLC-ELSD) was developed for the determination of sucralose in dairy foods. Sucralose was extracted by precipitating samples with 0.2% acetic acid solution, cleaned up on a C18 solid phase extraction cartridge and concentrated prior to HPLC-ELSD analysis. The relationship between logarithmic peak area and logarithmic sucralose concentration was linear over the range of 0.1 to 0.8 g/L, with a correlation coefficient (R2) of 0.9991. The recoveries of sucralose from spiked milk powder and liquid milk ranged between 94% and 102%. The limit of detection was 2 mg/L (RSN = 3).