| 油菜核不育两用系不育基因RAPD标记研究初报 |
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| 引用本文： | 黄团,黄先群,李丽,彭慧元.油菜核不育两用系不育基因RAPD标记研究初报[J].西南农业学报,2009,22(6). |
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| 作者姓名： | 黄团 黄先群 李丽 彭慧元 |
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| 作者单位： | 1. 贵州省生物技术研究所,贵州,贵阳,550006;贵州省农业生物技术重点实验室,贵州,贵阳,550006
2. 贵州省生物技术研究所,贵州,贵阳,550006 |
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| 基金项目： | 贵州省科技攻关项目，贵州省科技攻关项目 |
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| 摘 要： | 为建立有效的不育基因分子标记辅助育种平台,以5个核不育两用系为研究材料,选用20条随机引物,利用RAPD混合集群分离分析法,对各两用系可育与不育基因池进行分子标记的初步分析.结果表明,在油研10号筛选到2个与不育基因相关的标记:BA2084-600和S1354-1300;在萝×诸中筛选到1个与不育基因相关的标记:LC02-500.在827AB、T18筛选到集团间有差异的引物,未筛选到通过集团单株检测的与其育性基因相关的标记.通过RAPD混合BSA法可以筛选到与不育基因相关的特异DNA标记.
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| 关 键 词： | 核不育两用系 不育基因 分子标记 |
Preliminary Study on Male Sterile Gene of Dual Purpose Lines with Nucleic Male Sterile Gene by RAPD Molecular Marker in Brassica napus L. |
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| HUANG Tuan,HUANG Xian-qun,LI Li,Peng Hui-yuan.Preliminary Study on Male Sterile Gene of Dual Purpose Lines with Nucleic Male Sterile Gene by RAPD Molecular Marker in Brassica napus L.[J].Southwest China Journal of Agricultural Sciences,2009,22(6). |
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| Authors: | HUANG Tuan HUANG Xian-qun LI Li Peng Hui-yuan |
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| Abstract: | The molecular markers of fertile and sterile gene pool of 5 dual purpose lines with nucleic male sterile gene were analyzed by using 20 random primers to establish the supplemental breeding platform of effective sterile gene molecular markers. The results showed that two markers of BA2084-600and S1354-1300 related to sterile genes were selected from Youyan 10, the marker of LC02-500 related to sterile genes was selected from Raphnus sativus×Orychophragmus violaceus, and the different primers were selected from 827AB and T18 but the markers related to fertile genes were not selected from 827AB and T18. The specific DNA markers related to sterile genes could be selected by using the combination method of RAPD and BSA. |
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| Keywords: | RAPD Dual purpose line with nucleic male sterile line Sterile gene Molecular marker RAPD |
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