| 高效液相色谱法测定环丙氨嗪的含量 |
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| 引用本文： | 蒋成君.高效液相色谱法测定环丙氨嗪的含量[J].中国兽药杂志,2010,44(4):40-42. |
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| 作者姓名： | 蒋成君 |
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| 作者单位： | 1. 浙江省农产品化学与生物加工技术重点实验室,杭州,310023
2. 浙江新东港药业股份有限公司,浙江台州,318000 |
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| 摘 要： | 建立了环丙氨嗪含量测定的高效液相色谱方法。采用Phenomenex Prodigy C18色谱柱(250mm×4.6mm,5μm),甲醇:乙腈:缓冲液(每1000mL水中加3.72g磷酸氢二钾和6.48g磷酸二氢钾)=5:2:93为流动相,流速为2.0mL/min,检测波长214nm,柱温20℃。该方法线性范围为0.00799～0.01229mg/mL,r=0.99,系统精密度RSD=0.25%(n=6),平均回收率为99.2%(RSD=0.82%)。
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| 关 键 词： | 高效液相色谱法 环丙氨嗪 含量测定 |
| 收稿时间： | 5/7/2009 12:00:00 AM |
| 修稿时间： | 2009/5/14 0:00:00 |
Determination of Cyromazine by HPLC |
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| jiangchengjun.Determination of Cyromazine by HPLC[J].Chinese Journal of Veterinary Drug,2010,44(4):40-42. |
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| Authors: | jiangchengjun |
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| Institution: | JIANG Cheng-jun,XIE Tai-yang,TIAN Lian-ying (1.Key Laboratory of Agricultural Products Chemical , Biological Processing Technology of Zhejiang Province,Hangzhou 310023,2.Zhejiang Neo-Dankong Pharmaceutical Co.Ltd.,Taizhou,Zhejiang 318000,China) |
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| Abstract: | The HPLC method was established for the determination of cyromazine. A chromatographic column of Phenomenex Prodigy C18(250×4.6 mm, 5 m) was used, with the mobile phase of methanol-acetonitrile-buffer solution (5:2:93) (every 1000 ml water add 3.72 g dipotassium hydrogen phosphate and 6.48g potassium dihydrogen) at the folw rate of 2.0 mLmin-1 and the detection wavelength of 214nm. The linear concentration was within the range of 0.00799~0.01229 mg/ml(r=0.99), RSD of system precision was 0.25% (n=6), and the average recovery was 99.2%(RSD=0.82%). This method was suitable to determine the content of cyromazine. |
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| Keywords: | HPLC cyromazine assay |
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