口蹄疫病毒灭活疫苗中VP1基因RNA抽提方法的建立

本试验提取疫苗种毒、BEI灭活病毒和白油乳化灭活疫苗病毒中的RNA，并就VP1基因进行了RT-PCR扩增、核酸序列测定和序列比对分析。结果显示，3种来源的VP1基因片段长度均为813 bp，与预期的目标相符，核苷酸序列显示3种方法处理的病毒VP1同源性为100%。说明灭活剂BEI和乳化工艺没有全部破坏口蹄疫病毒VP1基因核酸，为检测疫苗毒株的核酸序列建立了一种方法。

The Isolation Method Establishment of VP1 Gene RNA in Inactivated Vaccine against Foot-and-Mouth Disease

Inactivated vaccines against foot and mouth disease gathered from different manufacturers were screened by various methods.Gene VP1 RNA in live virus, inactivated vaccine and inactivated virus were isolated, RT PCR amplified, cloned, cDNA sequenced and comparative analysis. The results indicate that,VP1 RNAs isolated from live virus, virus inactivated by BEI and inactivated vaccine emulsified by white oil access to all of the 813 bp specific objectives of fragments, and the expected 813 bp fragment match. Nucleotide sequence revealed that  VP1 homology of the virus was 100% isolated by three methods. BEI and the emulsification process do not damage whole RNA structure of VP1. The isolated method to RNA can be used to analyse sequence of VP1 RNA, which derived from either live or inactivated Foot and mouth Disease virus.