| 草地早熟禾植株再生体系建立的初步研究 |
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| 引用本文： | 段碧华,韩宝平,高遐虹,段建平.草地早熟禾植株再生体系建立的初步研究[J].北京农学院学报,2006,21(4):20-24. |
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| 作者姓名： | 段碧华 韩宝平 高遐虹 段建平 |
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| 作者单位： | 北京农学院,植物科学技术系,北京,102206;北京农学院,植物科学技术系,北京,102206;北京农学院,植物科学技术系,北京,102206;北京农学院,植物科学技术系,北京,102206 |
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| 基金项目： | 国家高技术研究发展计划(863计划)
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北京市科技新星计划项目 |
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| 摘 要： | 为培育优良抗性品种,选取新歌来德(Nuglade)、自由Ⅲ(Freedom Ⅲ)和优异(Merit)3个草地早熟禾品种的不同外植体,在不同浓度的6-BA,KT和NAA的培养基上进行植株培养,研究其对愈伤组织诱导率、绿苗分化率及生根的影响。结果表明:在不同培养基上,不同外植体愈伤组织的诱导率有较大的差异。草地早熟禾种子的愈伤组织诱导和继代培养基以MS(有机)+B5(无机)+2 mg/L 2,4-D+0.4 mg/L 6-BA为最佳;分化培养基以MS(有机)+B5(无机)+1.0 mg/L KT或MS(有机)+B5(无机)+1.0 mg/L 6-BA为最佳;生根培养基以1/2(MS(有机)+B5(无机))+0.8 mg/L NAA最佳。初步建立起草地早熟禾的植株再生体系。
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| 关 键 词： | 草地早熟禾 外植体 植株再生体系 |
| 文章编号： | 1002-3186(2006)04-0020-05 |
| 收稿时间： | 2006-07-03 |
| 修稿时间： | 2006-08-03 |
Preliminary Studies on Plant Regeneration System of Poa pratensis L. |
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| DUAN Bi-hua,HAN Bao-ping,GAO Xia-hong,DUAN Jian-ping.Preliminary Studies on Plant Regeneration System of Poa pratensis L.[J].Journal of Beijing Agricultural College,2006,21(4):20-24. |
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| Authors: | DUAN Bi-hua HAN Bao-ping GAO Xia-hong DUAN Jian-ping |
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| Institution: | Department of Plant Sciences and Technology,Beijing Agricultural College, Beijing 102206,China |
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| Abstract: | The different explants of Poa pratensis L.cv.(Nuglade,Merit and Freedom Ⅲ) were cultivated on the different medium with 6-BA,KT and NAA.The effect of different medium on the frequency of callus induction,green plant,roots were studied.A preliminary plant regeneration system of Poa pratensis L.was established.The result showed that:the frequency of callus induction for three explants was different.The best medium for callus induction of seeds was MS(organic)+B5(inorganic)+2 mg/L 2,4-D+0.4 mg/L 6-BA,seedling differentiation was MS(organic)+B5(inorganic)+0.9 mg/L KT.and rhizogenesis was 1/2(MS(organic)+B5(inorganic))+0.8 mg/L NAA for Poa pratensis L. |
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| Keywords: | Poa pratensis explants plant regeneration system |
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