| 11株草茎点霉培养特征、致病力及RAPD分析 |
| |
| 引用本文： | 谷祖敏,纪明山,魏松红,祁之秋,王英姿.11株草茎点霉培养特征、致病力及RAPD分析[J].吉林农业大学学报,2010,32(2):140-144. |
| |
| 作者姓名： | 谷祖敏 纪明山 魏松红 祁之秋 王英姿 |
| |
| 作者单位： | 沈阳农业大学植物保护学院,辽宁省生物农药工程技术研究中心,沈阳110161 |
| |
| 基金项目： | 辽宁省“十五”科技攻关项目(2006215004);;辽宁省工程技术研究中心专项(200535) |
| |
| 摘 要： | 从辽宁的沈阳、抚顺、鞍山等地采集的鸭跖草叶斑病病叶上共分离得到11个菌株。这些菌株在培养形态、生长速率、产孢能力、致病能力和产毒能力方面差异显著。其中SY1菌株的生长速率最快,SY4菌株的产孢能力最强。SYAU-06菌株的致病力最强,接种在鸭跖草叶片上发病率达到97.33%,病情指数为87.23,显著高于其他菌株。在液体培养基中SYAU-06菌株的产毒量最多,毒素的致病性也最强,在鸭跖草叶片上可形成面积为13.63 mm2的病斑。11个菌株基因组DNA经RAPD分析表明,不同菌株间扩增图谱差别明显,表现出各菌株间明显的遗传多态性。经聚类分析显示草茎点霉不同菌株的RAPD类群与致病力有一定的相关性。
|
| 关 键 词： | 草茎点霉 培养特性 致病能力 产毒能力 RAPD |
| 收稿时间： | 2009-03-23 |
| 修稿时间： | 2009-11-05 |
Analysis of the Cultural Characteristics,Pathogenicity and RAPD of 11 Phoma herbarum Isolations |
| |
| GU Zu-min,JI Ming-shan,WEI Song-hong,QI Zhi-qiu,WANG Ying-zi.Analysis of the Cultural Characteristics,Pathogenicity and RAPD of 11 Phoma herbarum Isolations[J].Journal of Jilin Agricultural University,2010,32(2):140-144. |
| |
| Authors: | GU Zu-min JI Ming-shan WEI Song-hong QI Zhi-qiu WANG Ying-zi |
| |
| Institution: |
College of Plant Protection, Shenyang Agricultural University, Biopesticide Engineering Research Center of Liaoning Province| Shenyang 110161, China |
| |
| Abstract: | Eleven isolations of Phoma herbarum were obtained from diseased leaves collected from Shenyang,Fushun,Anshan and other regions in Liaoning province.There were significant differences in cultural characteristics,growth speed,sporulation capacity,pathogenicity and toxin-producing capability among different isolations.In all isolations SY1 grew most rapidly and SY4 produced most spores. SYAU-06 strain had most virulence of which diseased leaf rate was 97.33% and disease index was 87.23 after being inoculated i... |
| |
| Keywords: | Phoma herbarum cultural characteristic pathogenic ability toxin-producing capability RAPD |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
| 点击此处可从《吉林农业大学学报》浏览原始摘要信息 |
| 点击此处可从《吉林农业大学学报》下载免费的PDF全文 |
|
