| 柱前衍生化HPLC法测定铁皮石斛破壁饮片中甘露糖的含量 |
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| 引用本文： | 晏薇娜,乔杨,张敏,田民义,王慧娟.柱前衍生化HPLC法测定铁皮石斛破壁饮片中甘露糖的含量[J].贵州大学学报(农业与生物科学版),2017(2):90-94. |
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| 作者姓名： | 晏薇娜 乔杨 张敏 田民义 王慧娟 |
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| 作者单位： | 1. 贵州大学药学院,贵州 贵阳550025;贵州省药食同源植物资源开发工程技术研究中心,贵州 贵阳550025;贵州省药食两用资源应用开发工程实验室,贵州 贵阳550025;2. 黔西南民族职业技术学院,贵州兴义,562400;3. 贵州省药食同源植物资源开发工程技术研究中心,贵州 贵阳550025;贵州省药食两用资源应用开发工程实验室,贵州 贵阳550025 |
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| 基金项目： | 贵州省药食同源植物资源开发工程技术研究中心(黔科合G字[2015]4001号)，贵州省药食两用资源应用开发工程实验室(黔发改投资[2015]542号)，贵州省中药材、民族药材质量标准研究课题任务书 |
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| 摘 要： | 采用柱前衍生化HPLC法测定了12批铁皮石斛破壁饮片中甘露糖的含量。药材采用80%乙醇回流提取除杂后,水煎煮制得石斛多糖,然后将多糖经酸水解为单糖,再用1-苯基-3-甲基-5-吡唑啉酮(PMP)进行衍生化处理,以盐酸氨基葡萄糖作为内标进样测定。结果平均校正因子为0.8248,RSD为0.63%,平均加样回收率为98.03%。每批铁皮石斛破壁饮片中单糖含量存在明显差异,但均符合药典要求。
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| 关 键 词： | 柱前衍生化 铁皮石斛 甘露糖 HPLC 含量测定 |
Determination of Mannose Content in Decoction Pieces of Wall-broken Dendrobium by Pre-column Derivatization HPLC Method |
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| YAN Wei-na,QIAO Yang,ZHANG Min,TIAN Min-yi,WANG Hui-juan.Determination of Mannose Content in Decoction Pieces of Wall-broken Dendrobium by Pre-column Derivatization HPLC Method[J].Journal of Mountain Agriculture & Biology,2017(2):90-94. |
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| Authors: | YAN Wei-na QIAO Yang ZHANG Min TIAN Min-yi WANG Hui-juan |
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| Abstract: | Pre-column derivatization HPLC method was adopted to measure mannose content in 12 batches of Dendrobium.After removing impurity by reflux extraction with 80％ ethanol,Dendrobium polysaccharides of the medicinal materials were prepared by water decoction,and then hydrolyzed into monosaccharides which was treated with 1-phenyl-3-methyl-5-(PMP).Glucosamine hydrochloride was used as the internal standard for sample determination.The results showed that the average correction factor was 0.8248,RSD was 0.63％,and the average recovery was 98.03％.Although significant differences in the content of monosaccharide in each batch of medicinal materials were observed,they all met the pharmacopoeia requirements.. |
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| Keywords: | pre-column derivatization dendrobium mannose HPLC determination of content |
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