拟态弧菌OmpU抗原表位的预测与多表位疫苗分子的设计

以拟态弧菌安徽分离株ＯｍｐＵ基因序列为基础，应用ＤＮＡＳｔａｒＰｒｏｔｅａｎ软件联合采用多种方法对ＯｍｐＵ的二级结构、柔性、亲水性、表面可能性和抗原指数等方面进行分析，预测ＯｍｐＵ的抗原表位，并以此设计多表位疫苗分子序列。结果表明，拟态弧菌ＯｍｐＵ至少含有９个Ｂ细胞表位，其中第２４～３１、５６～６６、９３～１１１、１２３～１２９、 １８３～１９８、２５３～２５８和２７５～２８７共７个区段的平均抗原指数较高。使用ＡＡＹ接头以ｅｐｉｔｏｐｅ５－ｅｐｉｔｏｐｅ７－ｅｐｉｔｏｐｅ２－ｅｐｉｔｏｐｅ６－ｅｐｉｔｏｐｅ３－ｅｐｉｔｏｐｅ４－ｅｐｉｔｏｐｅ１排列方式串联的多表位序列最接近原蛋白构象，各表位间相对独立，抗原性参数也较好，可作为研制多表位疫苗的候选分子序列。

The Prediction of Antigenic Epitope in OmpU of V.mimicus and Molecular Designing of Multiepitope Vaccine

In order to predict the antigenic epitopes in OmpU and design multi - epitope vaccine molecular, the secondary structure ,flexibility, hydrophilicity, sueface probabity and antigenicity index of OmpU were analysed and by DNAStar Protean software basing on OmpU sequence of V. mimicus isolated from Anhui Province. The results showed that there were at least nine B -cell epitopes in OmpU of V. mimicus, the average antigenicity indexs were higher at 24 -31,56 -66,93 -111 ,123 - 129,183 - 198,253 - 258 and 275 - 287 7 domains. Multi - epitope sequence of epitope5 - epitope7 - epitope2 - epitope6 - epitope3 - epitope4 - epitopel in series connected by AAY was the closest to the original sequence of protein conformation, because of the relatively independent of each epitope and the better antigenicity parameters. So, it could be developed as a multi - epitope vaccine candidate molecular.