马齿苋多糖对雏鸡胸腺免疫功能的影响及其机制

为了观察马齿苋多糖(Portulace oleracea polysaccharide,POP)对雏鸡胸腺免疫功能的影响,并探讨其可能的作用机制,试验通过对雏鸡灌注不同剂量的POP,测定雏鸡胸腺指数,应用MMT(四甲基偶氮唑盐比色法)方法测定雏鸡胸腺淋巴细胞转化率,利用流式细胞器测定雏鸡胸腺淋巴细胞周期与胸腺T淋巴细胞亚群的变化.结果表明,POP能显著增加雏鸡胸腺淋巴细胞转化率和胸腺指数(P＜0.05),淋巴细胞增殖指数显著提高(P＜0.05),CD4+T淋巴细胞含量显著增加(P＜0.05),CD8+T淋巴细胞数量变化不明显,CD4+/CD8+T淋巴细胞比值显著升高(P＜0.05).POP可通过调节雏鸡胸腺内细胞水平的变化,增强雏鸡细胞免疫功能,促进免疫系统发育.

Effects of Portulace oleracea Polysaccharide on Thymus Immune Function in Chicken and Its Mechanism

To observe the effect of POP(Portulace oleracea polysaccharide) on the change of thymus immune funtion in chicken,and study its mechanism,40 chickens were randomly allocated to 4 groups and fed with POP of different level.And then the thymus indexes,the thymus transformation rate of T lymphocyte and the change of T lymphocyte and thymus cell period were measured by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide colorimetric method(MTT) and flow cytometry.The results showed that the thymus indexes,transformation rate of lymphocyte,lymphocytes proliferation indices(LPI),and the CD4+T lymphocyte of POP groups were significantly higher than the control group(P<0.05).There was no significant change in CD8+T lymphocyte;and the ratio between CD4+/CD8+T lymphocyte was increased(P<0.05).It was indicated that POP could enhance the immune function and immunity in chicken by adjusting the cell level changes in thymus.