| 大花蕙兰原球茎的诱导、增殖及植株再生研究 |
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| 引用本文： | 季华,吴伟剑,吴华,陈龙清.大花蕙兰原球茎的诱导、增殖及植株再生研究[J].湖北农业科学,2012,51(15):3369-3371,3405. |
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| 作者姓名： | 季华 吴伟剑 吴华 陈龙清 |
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| 作者单位： | 1. 华中农业大学园艺林学学院/园艺植物生物学教育部重点实验室,武汉,430070
2. 武汉市黄陂区武湖农场,武汉,430345 |
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| 基金项目： | 中央高校基本科研业务费专项资金(2010QC028);华中农业大学硕士研究生创新研究项目(3003-457);湖北省科技攻关重大项目(2006AA205A04) |
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| 摘 要： | 从大花蕙兰(Cymbidium hybridum)试管苗取材,诱导原球茎,建立植株再生体系,分析了不同激素及其浓度对原球茎诱导、增殖和植株生根的影响.结果表明,不定芽比叶片更容易诱导出原球茎；生长素NAA对原球茎的诱导效果好于2,4-D,最适合原球茎诱导的激素组合为1.0 mg/L BA+1.0 mg/L NAA;原球茎增殖培养基中添加1.0 mg/L BA+0.5 mg/L NAA增殖效果较好,增殖系数可达7.90;最适生根培养基为1/2 MS+0.5 mg/L NAA+30 g/L蔗糖.
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| 关 键 词： | 大花蕙兰(Cymbidium hybridum) 原球茎 组织培养 植株再生 |
Study on Protocorm Induction,Proliferation and Plantlet Formation of Cymbidium hybridum |
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| JI Hua
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WU Wei-jian
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WU Hua
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CHEN Long-qing.Study on Protocorm Induction,Proliferation and Plantlet Formation of Cymbidium hybridum[J].Hubei Agricultural Sciences,2012,51(15):3369-3371,3405. |
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| Authors: | JI Hua WU Wei-jian WU Hua CHEN Long-qing |
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| Institution: | 1(1.Key Laboratory of Hoticultural Plant Biology,Ministry of Education/ College of Horticulture and Forestry,Huazhong Agricultural University,Wuhan 430070,China;2.Wuhu Farm,Huangpi District,Wuhan 430345,China) |
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| Abstract: | Explants were drawing from Cymbidium hybridum plantlet for protocorm induction and plantlet regeneration.Effects of the kind and concentration of plant growth regulators on protocorms induction,proliferation and root formation were analyzed.The results showed that introducing protocorm from adventitious bud was more easily than from leaf primordial.NAA was more effective for protocorm induction than 2,4-D.The optimum hormone combination for protocorm induction was 1.0 mg/L BA+1.0 mg/L NAA.The multiplication coefficient of protocorm was the highest(7.90) in medium adding 1.0 mg/L BA+0.5 mg/L NAA.The optimal medium for root formation was 1/2 MS+0.5 mg/L NAA+ 30 g/L sucrose. |
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| Keywords: | Cymbidium hybridum protocorm tissue culture plant regeneration |
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