树莓果实维生素C积累与其合成氧化酶的关系

对4个品种的树莓(Rubus idaeus)果实生长过程中还原型维生素C(AsA)、氧化型维生素C(DHA)及总维生素C舍量进行了分析,并对L-半乳糖酸-1,4-内酯脱氢酶(GalLDH)、抗坏血酸氧化酶(AAO)及抗坏血酸过氧化物酶(APX)3种酶的活性进行了测定分析.结果显示,AsA和总维生素C含量随着果实的成熟而逐渐上升.4个品种中GalLDH活性与AsA积累之间均呈显著正相关.AAO和APX活性与AsA含量相关性不显著,而APX活性与DHA含量呈显著正相关.说明不同树莓品种间维生素C含量存在差异的原因与其GalLDH及APX活性有关.

Relationship between Vitamin C Accumulation and Synthase or Oxidase Activities of Rubis idaeus Fruit

The ascorbic acid(AsA),Dehydroascorbic acid(DHA) and total vitamin C content of four raspberry(Rubus idaeus) varieties were tested during fruit development;and enzyme activities of L-galactono-1,4-lactone dehydrogenase(GalLDH),ascorbic acid oxidase(AAO) and ascorbate peroxidase(APX) were also measured.The result showed that the content of AsA and vitamin C continually accumulated with the fruit development.GalLDH activity was highly significant positive correlated to ASA accumulation in the four varieties.AAO and APX activity had no significant correlation to AsA;but APX activity was significantly positively correlated to DHA content,which indicated that the difference of vitamin C content in raspberry varieties was related to GalLDH and APX activity.