| 利用SCAR-PCR检测杂交油菜"蜀杂9号"的杂种纯度 |
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| 引用本文： | 张帆,李江,李熠毅,李小艳,刘璐,王茂林,赵云.利用SCAR-PCR检测杂交油菜"蜀杂9号"的杂种纯度[J].西南农业学报,2006,19(1):32-34. |
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| 作者姓名： | 张帆 李江 李熠毅 李小艳 刘璐 王茂林 赵云 |
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| 作者单位： | 1. 四川省种子管理站,四川,成都,610041
2. 四川大学生命科学学院,四川,成都,610064 |
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| 基金项目： | 国家863项目（2001AA241104）“优质、高产、抗病甘蓝型油莱新杂交种培育” |
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| 摘 要： | 用两个分别能在“蜀杂9号”父、母本之间稳定产生多态性的随机引物S18和S31,对父、母本DNA样品进行RAPD扩增,将得到的特异标记片段S18750和S31550进行回收、克隆和测序。根据测序结果设计序列特异性扩增(SCAR)引物SZ9SP1和SZ9SP2,将“蜀杂9号”的亲本RAPD标记转化为序列特异的SCAR标记。当用两对引物分别扩增杂种F1代单株总DNA时,能分别获得父、母本特异序列扩增带,表明获得的SCAR标记能可靠地用于“蜀杂9号”杂种纯度的检测。
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| 关 键 词： | 杂交油菜 杂种纯度 分子标记 RAPD SCAR |
| 文章编号： | 1001-4829（2006）01-0032-03 |
| 收稿时间： | 2005-07-12 |
| 修稿时间： | 2005-07-12 |
Development of SCAR markers to authenticate the hybrid seed purity of Shuza No.9 (Brassica napus L.) |
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| ZHANG Fan,LI Jiang,LI Yi-yi,LI Xiao-yan,LIU Lu,WANG Mao-lin,ZHAO Yun.Development of SCAR markers to authenticate the hybrid seed purity of Shuza No.9 (Brassica napus L.)[J].Southwest China Journal of Agricultural Sciences,2006,19(1):32-34. |
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| Authors: | ZHANG Fan LI Jiang LI Yi-yi LI Xiao-yan LIU Lu WANG Mao-lin ZHAO Yun |
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| Institution: | 1. Siehuan Provincial Seed Station,Sichuan Chengdu 610041, China; 2. College of Life Science, Siehuan Univesity, Siehuan Chengdu 610064, China |
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| Abstract: | Two parental-specific DNA bands S18_(750) and S31_(550) amplified with parental DNA pools of hybrid rapeseed 'Shuza No.9' by RAPD primers S18 and S31 were cloned and sequenced,respectively.Two pairs of primers SZ9SP1 and SZ9SP2 were designed according to the sequences of S18_(750) and S31_(550) and the parents SCAR-PCR markers were obtained.When the two pairs of designed primers were used to amplify DNAs of individual plant in F_1 hybrids,respectively,maternal/ paternalspecific band could be appeared in all F_1 hybrids,indicating that the SCAR markers could reliably be used to authenticate the hybrid seed lots of Shuza No.9. |
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| Keywords: | Brassica napus L hybrid seed purity molecular marker RAPD SCAR |
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