盐析法提取鹅血清中G型免疫球蛋白工艺条件的优化

利用盐析法对鹅血清中的免疫球蛋白进行分离提取,得到免疫球蛋白的粗提物。根据单因素的实验结果,通过四因素五水平二次旋转正交组合实验设计,鹅血清中免疫球蛋白的最佳提取工艺条件为：提取温度34℃,静止时间58min,血清-PBS比例2∶1,缓冲溶液pH7.2,此时的IgG提取量达到0.681mg·mL^-1。通过方差分析和验证实验,数学模型可靠并且拟和较好。

Optimization of Extraction Technique of lgG in Goose Serum by Saturated Ammonium Sulphate Method

In this article, the Ig was isolated from goose serum by the neuter salt methods using saturated ammonium sulphate, and the crude extraction then was got. Based on the results of single-factor experiments, using the method of orthogonal rotation combination test design method to four factors and five levels, the extraction method of IgG from goose serum was studied. The optimal parameters of extracting technology were as follow： extracting temperature was 34℃, time of digestion 60min, mixing serum and PBS at 2：1 and buffer solution for pH 7.2 and the extraction rate of IgG reached 0.681 mg/mL. Through the variance analysis and verification test ,it was showed that mathematical model was reliable and the fitting of the model was better.