| 琯溪蜜柚果肉过氧化物酶cDNA的克隆及序列分析 |
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| 作者姓名： | 林琳 郭志雄 潘东明 |
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| 作者单位： | 福建农林大学园艺学院 福州350002(林琳，郭志雄)，福建农林大学园艺产品贮藏保鲜研究所 福州350002(潘东明) |
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| 基金项目： | 2004年度高等学校博士学科点专项科研基金课题“琯溪蜜柚汁胞粒化过程中基因差别表达的研究”（20040389008）;福建省跨越计划项目跨越计划项目“琯溪蜜柚汁胞粒化症矫治技术研究与示范推广”（闽财指[2002]465号）. |
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| 摘 要： | 以琯溪蜜柚果肉为材料,运用cDNA克隆的方法,克隆得到了琯溪蜜柚果肉过氧化物酶的cDNA的全长,并对得到的序列进行了一些分析,从分子生物学角度对粒化的研究做了初步的探索。结果表明:琯溪蜜柚果肉PODcDNA全长1263bp,有完整的阅读框,编码351个氨基酸。另外5''非翻译区42bp,3''非翻译区168bp(不包含终止密码子TAA),其中包括一个多聚腺苷酸化信号AATAAA,以及一个含24个腺苷酸的poly(A)尾。由琯溪蜜柚果肉PODcDNA推导的氨基酸序列与无花果(Fi-cuscarica)、陆地棉(Gossypiumhirsutum)、杨属植物(Populus)等同源性较高,分别为:58.6%,67.61%,65.24%,67.14%等。
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| 关 键 词： | 琯溪蜜柚 过氧化物酶 cDNA 序列分析 同源性分析 |
Cloning and Sequence analysis of the Citrus grandis (L.) Osbeck Flesh Peroxidase cDNA |
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| Authors: | Lin Lin Guo Zhixiong Pan Dongming |
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| Institution: | 1.College of Horticulture, Fujian Agriculture and Forestry University, Fuzhou 350002; 2.Institute of Storage Science and Technology of Horticultural Products, Fujian Agriculture and Forestry University, Fuzhou 350002 |
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| Abstract: | The pummeloCitrus grandis (L.) Osbeck] Flesh Peroxidase(POD) cDNA was cloned, sequenced and analysised. The results showed that the complete cDNA was 1263bp in length, including 42bp of 5'' untranslatable region, 168bp of 3'' untranslatable region which contained a polyadenylation signal, AATAAA, and a 24bp polyadenylation tail. The coding region encoded a peptide of 588 amino acid residues. The deduced amino acid sequence had a significant homology to the POD sequences from fig (Ficus carica), upland cotton (Gossypium hirsutum) and poplar (Populus), were 58.6%, 67.61%, 65.24%, 67.14%, respectively. |
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| Keywords: | Pummelo Peroxidase cDNA Sequencing homologous analysis |
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