| 显齿蛇葡萄离体快繁体系的建立 |
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| 引用本文： | 张霞,杨朝东,倪德江.显齿蛇葡萄离体快繁体系的建立[J].安徽农业科学,2011,39(17):10157-10159,10166. |
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| 作者姓名： | 张霞 杨朝东 倪德江 |
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| 作者单位： | 长江大学园艺园林学院,湖北,荆州,434025;华中农业大学园艺林学学院,农业部亚热带农业资源与环境重点开放实验室,湖北,武汉,430070 |
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| 基金项目： | 黑龙江自然科学基金项目(C200620) |
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| 摘 要： | 目的]利用组织培养的方法建立快速、高效的显齿蛇葡萄快速繁殖体系。方法]以显齿蛇葡萄带芽茎段为材料，研究了不同灭菌方法、培养基和激素组合对显齿蛇葡萄离体快繁的影响。结果]外植体采用75％乙醇浸泡20s＋0．1％HgCl2处理8min＋吐温-801-2滴灭菌效果较好；腋芽诱导适宜的培养基为B5＋1．00mg／LBA＋0．05mg／LNAA；增殖培养以MS／B5＋1．50mg／LBA＋0．05mg／LNAA为宜；生根以1／2MS＋O．50mg／LIBA的培养基效果好。结论]建立了一个显齿蛇葡萄离体快繁的高频率发生体系，为其愈伤再生体系、遗传转化及无性系变异筛选奠定了技术基础。
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| 关 键 词： | 显齿蛇葡萄 外植体 离体培养 |
Establishment of Rapid Propagation System in vitro of Ampelopsis grossedentata |
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| ZHANG Xia et al.Establishment of Rapid Propagation System in vitro of Ampelopsis grossedentata[J].Journal of Anhui Agricultural Sciences,2011,39(17):10157-10159,10166. |
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| Authors: | ZHANG Xia |
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| Institution: | ZHANG Xia et al (College of Forestry,Northeast Forestry University,Harbin,Heilongjiang 150040) |
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| Abstract: | Objective] The ras promoters were cloned from genomic DNA of Auricuralia auricular so as to provide promoters for breeding better species by genetic technology.Method] PCR was used to clone promoters with the genomic DNA of A.auricular strain YBS-3 as template,and then the sequences of four ras promoters were obtained.The analysis of promoter sequences was made by three promoter analysis software:Promoter prediction,Place and TFSEARCH ver.1.3.Result] Four fragments contained core elements of promoter inc... |
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| Keywords: | Auricuralia auricular ras promoter Cloning Sequence analysis |
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