辐照对瓜实蝇遗传区性品系成虫肠道微生物的影响

为研究辐照对瓜实蝇肠道微生物群落多样性和结构组成的影响,以100 Gy137Cs辐照来自瓜实蝇遗传区性品系(GSS)的5、8、11和13日龄的雌、雄成虫,并采用Illumina MiSeq测序技术分别对其肠道菌群进行测序分析。结果表明,瓜实蝇雌雄成虫的肠道中存在19个主要的菌属,包括17个属名已知,1个属名未知和1个属不可培养。其中,17个已知属分别属于4个细菌门,即变形菌门(Proteobacteria)(占总量的50%~98%)、厚壁菌门(Firmicutes)(占总量的2%~47%)、拟杆菌门(Bacteroidetes)(占总量的1%~16%)和1个至今微生物界还未确定的稀少菌门(unclassified rare phyla)(总量1%)。此外,辐照降低了瓜实蝇肠道中的微生物多样性以及优势菌的丰富度,且这种影响与瓜实蝇的性别和成虫日龄无关。本研究结果为评估和修复辐照对瓜实蝇的损伤提供了参考。

Effect of Irradiation on Bacterial Communities in the Gut of Adult Bactrocera cucurbitae From the Genetic Sexing Strain (Diptera: Tephritidae)

In order to explore the influences of irradiation the diversity and structural composition of intestinal microflora in Bactrocera cucurbitae (Coquillett),adult females and males from B.cucurbitae genetic sexing strain (GSS) at different ages (5,8,11,13 d respectively) were irradiated by 137Cs at the dose of 100 Gy.Illumina MiSeq high-throughput sequencing platform was used to study the changes of gut microbial communities caused by irradiation.The results showed that 19 major bacteria genera were detected in adults'guts,of which 17 genera were known,and 1 was unclassified with 1 uncultured.The 17 known genera belong to 4 phyla,namely Proteobacteria (accounted for 50％ to 98％ of total amount),Firmicutes (accounted for 2％ to 47％ of total amount),Bacteroidetes (accounted for 1％ to 16％ of total amount) and one unclassified rare phyla (accounted for ＜ 1％ of total amount),respectively.In addition,the irradiation could significantly reduce the diversity and relative abundance of gut symbiotic bacteria in B.cucurbitae GSS adults,and the influences caused by irradiation had nothing to do with sex and adult ages.These results provide a reference for assessing and repairing the irradiation damage to B.cucurbitae.