| 苹果炭疽病病原菌ISSR-PCR反应体系的优化及遗传多样性分析 |
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| 引用本文： | 符丹丹,庄杰丽,张荣,孙广宇.苹果炭疽病病原菌ISSR-PCR反应体系的优化及遗传多样性分析[J].植物保护学报,2013,40(3):231-236. |
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| 作者姓名： | 符丹丹 庄杰丽 张荣 孙广宇 |
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| 作者单位： | 1. 西北农林科技大学植物保护学院,旱区作物逆境生物学国家重点实验室,陕西杨凌712100;河南科技大学食品与生物工程学院,洛阳471023
2. 西北农林科技大学植物保护学院,旱区作物逆境生物学国家重点实验室,陕西杨凌712100 |
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| 基金项目： | 国家自然科学基金 (31171797) |
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| 摘 要： | 为了从分子水平探讨苹果炭疽病病原菌的群体遗传多样性,采用L16 (45)正交设计对ISSRPCR反应体系中的Mg2浓度、dNTP浓度、Taq DNA聚合酶用量、引物浓度和DNA含量进行优化,并利用优化的体系进行引物筛选及苹果炭疽病菌遗传多样性分析.确立最优反应体系为2.0mmol/L Mg2+、0.2 mmol/L dNTP、2UTaqDNA聚合酶、1μmol/L引物和DNA 100 ng.筛选获得的10条引物对供试菌株共扩增出42条谱带,均为多态性条带.供试菌株在相似系数0.50处分为2个类群,分别与根据形态学鉴定的胶孢刺盘孢Colletotrichum gloeosporioides和尖孢刺盘孢C.acutatum 2个类群相一致,在相似系数0.74处,分为4个亚群,表明苹果炭疽病菌存在明显的种间及种内遗传分化.
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| 关 键 词： | 苹果炭疽病 胶孢刺盘孢 尖孢刺盘孢 种群 |
| 收稿时间： | 1/4/2013 12:00:00 AM |
Optimization of ISSR-PCR reaction system and genetic diversity analysis of Colletotrichum species causing bitter rot of apple |
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| Fu Dandan,Zhuang Jieli,Zhang Rong and Sun Guangyu.Optimization of ISSR-PCR reaction system and genetic diversity analysis of Colletotrichum species causing bitter rot of apple[J].Acta Phytophylacica Sinica,2013,40(3):231-236. |
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| Authors: | Fu Dandan Zhuang Jieli Zhang Rong and Sun Guangyu |
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| Institution: | State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China;College of Food & Bioengineering, Henan University of Science and Technology, Luoyang 471023, Henan Province, China;State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China;State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China;State Key Laboratory of Crop Stress Biology for Arid Areas, College of Plant Protection, Northwest A&F University, Yangling 712100, Shaanxi Province, China |
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| Abstract: | |
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| Keywords: | apple bitter rot Colletotrichum gloeosporioides Colletotrichum acutatum population |
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