| 莫能菌素人工抗原合成及其多克隆抗体的制备 |
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| 引用本文： | 魏瑞成,王冉,刘铁铮.莫能菌素人工抗原合成及其多克隆抗体的制备[J].江苏农业学报,2007,23(4):346-350. |
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| 作者姓名： | 魏瑞成 王冉 刘铁铮 |
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| 作者单位： | 江苏省畜禽产品安全性研究重点实验室,江苏省农业科学院食品质量安全与检测研究所,江苏,南京,210014 |
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| 摘 要： | 通过莫能菌素羟基与琥珀酸酐反应,合成半抗原莫能菌素-琥珀酰半酯,采用混合酸酐法将其与载体蛋白BSA偶联制备人工抗原,以此人工抗原免疫新西兰大白兔获得多克隆抗体.试验结果表明,莫能菌素结合抗原免疫家兔制备的抗体对莫能菌素具有很高的特异性、亲合性和选择性.用此抗体建立的莫能菌素间接竞争酶联免疫吸附检测方法的标准工作曲线I50值为37.9 ng/ml,最低检测限(I10)为2.09 ng/ml.
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| 关 键 词： | 莫能菌素 半抗原 酶联免疫吸附法(ELISA) 多克隆抗体 莫能菌素 人工抗原合成 多克隆抗体 Polyclonal Antibodies Preparation Antigen Artificial 最低检测限 工作曲线 标准 检测方法 酶联免疫吸附 间接竞争 选择性 亲合性 家兔 结合 结果 试验 新西兰大白兔 |
| 文章编号： | 1000-4440（2007）04-0346-05 |
| 修稿时间： | 2006-09-24 |
Synthesis of Artificial Antigen and Preparation of Polyclonal Antibodies against Monensin |
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| WEI Rui-cheng,WANG Ran,LIU Tie-zheng.Synthesis of Artificial Antigen and Preparation of Polyclonal Antibodies against Monensin[J].Jiangsu Journal of Agricultural Sciences,2007,23(4):346-350. |
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| Authors: | WEI Rui-cheng WANG Ran LIU Tie-zheng |
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| Institution: | Key Lab of Animal-derived Food Safety of Jiangsu Province, Institute of Food Quality Safety and Detection, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China |
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| Abstract: | To produce artificial antigens susceptible to raise antibodies against monensin,the monosuccinyl monensin synthesized through the reaction between succinate acid anhydride and monensin were coupled with bovine serum albumin(BSA)by the mixed acid anhydride reaction.The artificial antigen was then applied to immunize three New Zealand white rabbits.The polycolonal antiboldy obtained demonstrated high specificity,affinity and selectivity against monensin.The I50 of standard curves established between monensin and the antibody by indirect competitive ELISA was 37.9 ng/ml and minimum detection was 2.09 ng/ml. |
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| Keywords: | monensin hapten enzyme linked immunosorbent assay(ELISA) polyclonal antibody |
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