| 高效液相色谱法测定家兔血浆中丹参素的含量 |
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| 引用本文： | 梁岩冰,田玉琴.高效液相色谱法测定家兔血浆中丹参素的含量[J].河北北方学院学报(自然科学版),2012,28(2):68-69,72. |
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| 作者姓名： | 梁岩冰 田玉琴 |
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| 作者单位： | 1. 河北大学药学院,河北保定,071002
2. 河北北方学院,河北张家口,075000 |
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| 摘 要： | 目的建立测定家兔血浆中丹参素含量的方法.方法高效液相色谱法（HPLC）,色谱柱为：YMC-PACK ODS-A（150mm×4.6mm,5μm）,流动相为甲醇-0.5%冰醋酸（1∶8v/v）,检测波长281nm.血浆样品用液-液萃取法预处理.结果丹参素平均回收率为98.34%,RSD小于4%.结论此方法灵敏度高,操作简便,准确,适应于血浆样品的快速、大量分析.
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| 关 键 词： | 丹参素 血浆 含量测定 高效液相色谱法 |
Determination of Tanshinol in Rabbit Plasma by HPLC |
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| LIANG Yan-bing,TIAN Yu-qin.Determination of Tanshinol in Rabbit Plasma by HPLC[J].Journa of Hebei North University:Natural Science Edition,2012,28(2):68-69,72. |
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| Authors: | LIANG Yan-bing TIAN Yu-qin |
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| Institution: | 1.College of Pharmaceutical Science,Hebei University,Baoding 071002,Hebei,China; 1.Hebei North University,Zhangjiakou 075000,Hebei,China) |
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| Abstract: | Objective To establish a method for the determination of contents of Tanshinol in plasma.Methods The contents were determined by HPLC,with YMC-PACK ODS-A(150mm×4.6 mm,5μm),MeOH-0.5% glacial acetic acid(1∶8 v/v) as mobile phase and UV detector at 281 nm.Results The average recovery of Tanshinol was 98.34%.RSD was no more than 4%.Conclusion This method was rapid and simple,the result was stable and reproducible,and the separable effect was good.Thus the method could be used for determination of Tanshinol in plasma. |
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| Keywords: | Tanshinol plasma determination HPLC |
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