大花蕙兰再生体系建立的研究

目的]研究并建立大花蕙兰再生体系。方法]用75%酒精和0.1%升汞进行表面灭菌,以MS为基本培养基,采用L9(33)正交试验设计进行类原球茎增殖的优选,以继代培养40 d后的增殖系数及幼苗分化率为考察指标。结果]在大花蕙兰离体再生过程中,侧芽是最容易启动的外植体;MS+6-BA 1.0 mg/L+NAA 0.1 mg/L+AC 0.1 g/L是类原球茎诱导的最佳培养基;1/2 MS+6-BA 1.0 mg/L+NAA 0.1 mg/L是类原球茎增殖和幼苗分化的最适宜培养基;芽分化后,待长至2~3 cm时,小心切下接入1/2 MS+NAA 1.0 mg/L+GA31.0 mg/L+香蕉泥150 g/L培养基中生根壮苗效果最好。结论]为规模化生产提供依据。

Study on Establishment of Regeneration System of Cymbidium

Objective] The research aimed to study and establish the regeneration system of Cymbidium.Result] 75% alcohol and 0.1% were used to surface sterilization.The MS was used as basic culture medium.L9(33) orthogonal design was chose to optimize the protocorm-like body proliferation.The proliferation coefficient and the seedling differentiation rate after subcultured 40 d were chose as the index.Result] At in vitro propagation of the regeneration system built of cymbidium,axially bud was more easily started as explants.MS agar medium supplemented with 6-BA 1.0 mg/L+NAA 0.1 mg/L +AC 0.1 g/L was the optimal medium for the induction of protocor-like body.1/2 MS+6-BA 1.0 mg/L +NAA 0.1 mg/L was the best medium of protocor-like body multiplying and seedling differentiation.After seedling differentiation,in 1/2 MS medium supplemented with 1/2 MS+NAA 1.0 mg/L +GA3 1.0 mg/L + banana mud 150 g/L,the rooting and strong sprout of rootless bud could be accomplished efficiently and fast.Conclusion] The study provided the basis for scale productin.