| 利用卡那霉素筛选非洲菊转基因植株的技术研究 |
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| 引用本文： | 张宝琼,范眸天,屈云慧,于丽霞,李涵.利用卡那霉素筛选非洲菊转基因植株的技术研究[J].安徽农学通报,2009,15(3). |
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| 作者姓名： | 张宝琼 范眸天 屈云慧 于丽霞 李涵 |
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| 作者单位： | 云南省农科院花卉研究所,云南昆明,650205;云南农业大学,云南昆明,650201;云南农业大学,云南昆明,650201;云南省农科院花卉研究所,云南昆明,650205;西南林学院,云南昆明,650224 |
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| 基金项目： | 云南省攻关及高新技术发展计划，云南省科技计划项目 |
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| 摘 要： | 为了能够更高效地得到非洲菊转基因植株,将非洲菊的植株接种于含有不同浓度卡那霉素的分化培养基中,当卡那霉素浓度达到25 mg/L时,外植体的生长分化完全受抑制,苗白化.将非洲菊通过愈伤组织诱导,农杆菌介导得到转基因植株,把共培养3 d的外植体进行3种不同处理.结果表明,处理3较好,即先在培养基中直接加羧苄霉素不加卡那霉素,待苗长势较佳时,再用卡那霉素筛选,PCR检测,得到7棵阳性植株,再生率为43.75 %.既保证了芽诱导苗的成活率,又节省了大量提取DNA的时间和贵重药品的使用量.
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| 关 键 词： | 非洲菊 农杆菌 卡那霉素 |
Study on Selecting of Transgenic Gerbera Plants with kanamycin |
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| Zhang Baoqiong,et al..Study on Selecting of Transgenic Gerbera Plants with kanamycin[J].Auhui Agricultural Science Bulletin,2009,15(3). |
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| Authors: | Zhang Baoqiong |
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| Institution: | 1 Flower Research Insistute Of Yunnan Academy Agriculture Sciense;Kunming;650205;2 Yunnan Agriculture University;Kunming 650201;3 Southwest Forestry College;Kunming 650224 |
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| Abstract: | In order to gain more efficient Gerbera transgenic plants,Gerbera plants were inoculated with different concentrations of Km in the proliferation medium,The concention of Kanamycin at 25mg/L completely inhibited the explant growth and not shoot differentiation,young plant whitening.Through the Gerbera callus induction,Agrobacterium-mediated method to be transgenic plants,the co-cultured materials a total of 3-day explants for 3 to deal with in co-culture will be disseminated stage of the explant for 3 to de... |
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| Keywords: | Gerbera jamesonii Bolus Agrobacterium tumefaciens kanamycin |
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