| 蛹虫草多糖提取及纯化工艺研究 |
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| 引用本文： | 刘红锦,蒋宁,李建军,连云岚,张平.蛹虫草多糖提取及纯化工艺研究[J].江西农业学报,2007,19(12):80-82. |
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| 作者姓名： | 刘红锦 蒋宁 李建军 连云岚 张平 |
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| 作者单位： | 1. 江苏省农科院,农产品加工所,江苏,南京,210014
2. 山西省药品检验所,山西,太原,030001 |
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| 基金项目： | 江苏省农业科技攻关项目 |
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| 摘 要： | 采用以大米、蚕蛹为主原料的培养基培养蛹虫草子实体。通过正交实验对蛹虫草水溶性多糖的提取工艺进行了研究,利用蒽酮-硫酸法来测定多糖含量。发现90℃、加水20倍、分3次提取、每次3 h为最佳提取条件。同时考查了不同除蛋白方法对虫草多糖的蛋白去除效果。
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| 关 键 词： | 蛹虫草 多糖提取 脱蛋白 |
| 文章编号： | 11301-8581（2007）12-0080-03 |
| 收稿时间： | 2007-09-14 |
| 修稿时间： | 2007年9月14日 |
Study on Extraction and Purification of Polysaccharides from Cordyceps militaris |
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| LIU Hong-jing,JIANG Ning,LI Jian-jun,LIAN Yun-lan,ZHANG Ping.Study on Extraction and Purification of Polysaccharides from Cordyceps militaris[J].Acta Agriculturae Jiangxi,2007,19(12):80-82. |
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| Authors: | LIU Hong-jing JIANG Ning LI Jian-jun LIAN Yun-lan ZHANG Ping |
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| Abstract: | The medium mainly containing rice and the pupa of silkworm was used to cultivate the sporocarp of Cordyceps militaris.Extraction processing of water-soluble polysaccharide in Cordyceps militaris was studied through orthogonal experiment,and the anthrone-sulphuric acid method was used to determine polysaccharide content.Three times(3 hours per times) extraction with 20 times water at 90 ℃ was the best extraction conditions.Simultaneously deproteinization effect was examined with different methods. |
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| Keywords: | Cordyceps militaris Polysaccharide extraction Deproteinization |
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