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| 应用DNA芯片技术筛选禽致病性大肠杆菌和尿道致病性大肠杆菌的差异表达基因 | |
| 引用本文： | 宦海霞,张科,陈祥,高崧,刘秀梵.应用DNA芯片技术筛选禽致病性大肠杆菌和尿道致病性大肠杆菌的差异表达基因[J].中国预防兽医学报,2011,33(1). |
| 作者姓名： | 宦海霞 张科 陈祥 高崧 刘秀梵 |
| 作者单位： | 1. 淮阴师范学院,生命科学学院,江苏,淮安,223300;扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009 2. 淮安出入境检验检疫局,江苏,淮安,223300 3. 扬州大学,农业部畜禽传染病学重点开放实验室,江苏,扬州,225009 |
| 基金项目： | 国家自然科学基金(30972196、30771460、30471281、31001079); 国家“863”(2003AA222141); 江苏省高校自然科学研究项目(09KJB230002) |
| 摘 要： | 为研究禽致病性大肠杆菌强毒株E058的毒力相关基因在鸡体内、体外表达情况以及E058和尿道致病性大肠杆菌HEC4在LB和尿液中培养的表达情况,本研究分别提取E058株在SPF鸡体内及E058株和HEC4株在LB和尿液中静置培养的总RNA,与构建的DNA芯片杂交,检测和分析RNA的差异表达情况。芯片的检测结果表明:E058株在鸡体内和LB中培养差异表达基因共有9个,上调基因为5个,分别为neuC、iutA、cvaC、aes-15和iucCD;下调基因为4个,分别为aes-8、gyrB、aec-30和mdh。另外,芯片检测结果也显示E058株和HEC4株在LB和尿液中静置培养,具有相似的基因表达情况。 |
| 关 键 词： | 禽致病性大肠杆菌 尿道致病性大肠杆菌 毒力基因 基因表达差异 DNA芯片 |
DNA microarray analysis of differential gene expressions of avian pathogenic Escherichia coli and uropathogenic Escherichia coli in different growth conditions |
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| HUAN Hai-xia,ZHANG Ke,CHEN Xiang,GAO Song,LIU Xiu-fan.DNA microarray analysis of differential gene expressions of avian pathogenic Escherichia coli and uropathogenic Escherichia coli in different growth conditions[J].Chinese Journal of Preventive Veterinary Medicine,2011,33(1). | |
| Authors: | HUAN Hai-xia ZHANG Ke CHEN Xiang GAO Song LIU Xiu-fan |
| Institution: | HUAN Hai-xia1,2,ZHANG Ke3,CHEN Xiang2,GAO Song2*,LIU Xiu-fan2(1.College of Life Science,Huaiyin Normal University,Huaian 223300,China,2.Animal Infectious Disease Laboratory,Ministry of Agricultural,Yangzhou University,Yangzhou 225009,3.Huaian Entry-Exit Inspection and Quarantine Bureau,China) |
| Abstract: | To screen distinctively expressed genes of avian pathogenic Escherichia coli(APEC) O2 serotype E058 strain and uropathogenic Escherichia coli(UPEC) HEC4 strain,the mRNA from the E058 and HEC4 strains under different growth conditions were extracted and hybridized in DNA microarray chips,respectively.A total of 9 differential expressed genes of APEC E058 strain from inoculated chicken were identified when compared to those growth in LB broth,and five of them were up-regulated including icuCD,iutA,cvaC,neuC a... |
| Keywords: | avian pathogenic Escherichia coli uropathogenic Escherichia coli virulence gene distinctive gene expression DNA microarray |
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