| 对甜菜夜蛾高毒力Bt新菌株的PCR-RFLP分析及生物活性测定 |
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| 引用本文： | 程林友,陆秀君,李国勋.对甜菜夜蛾高毒力Bt新菌株的PCR-RFLP分析及生物活性测定[J].东北农业大学学报,2005,36(2):161-165. |
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| 作者姓名： | 程林友 陆秀君 李国勋 |
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| 作者单位： | 河北农业大学植物保护学院,河北,保定,071001;河北农业大学植物保护学院,河北,保定,071001;莱阳农学院,山东,青岛,266109 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 利用PCR-RFLP对Bt.新菌株cyz-13,T4-3的基因型进行分析。结果表明,cyz-13含有cry1Aa,cry1Ac,cry1Bc,cry2Ab4种基因型,其中cyz-13菌株的PCR产物的酶切片断类型不同于已知的基因类型;T4-3菌株含有cry1Aa,cry1Ac,cry1Ag,cry2Ac,cry1Ia5种基因型。室内生测结果显示,两菌株对甜菜夜蛾、粘虫、棉铃虫、玉米螟均具有高毒力,好于H D-1标准菌株。两菌株的发酵液均有杀虫活性,二者上清的杀虫因子不同。
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| 关 键 词： | 苏云金杆菌 cry基因 PCR-RFLP分析 生物测定 |
| 文章编号： | 1005-9369(2005)02-0161-05 |
| 修稿时间： | 2004年4月19日 |
PCR-RFLP analysis of new Bt strains against Spodoptera exigua and toxicity activity analysing |
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| CHENG Lin-you,LU Xiu-jun,LI Guo-xun.PCR-RFLP analysis of new Bt strains against Spodoptera exigua and toxicity activity analysing[J].Journal of Northeast Agricultural University,2005,36(2):161-165. |
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| Authors: | CHENG Lin-you LU Xiu-jun LI Guo-xun |
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| Abstract: | PCR-RFLP was used for detecting the cry-type genes from new Bacillus thuringiensis strains, T4-3 and cyz-13. The results showed that there was five cry-type genes including cry1Aa, cry1Ac, cry1Ag, cry2Ac and cry1Ia in Bt T4-3 strains; Three types of gene of cry1Ac cry1Bc cry2Ab were identified in Bt cyz-13 strain, The result of restriction enzyme analysis showed that the fragments from cyz-13 strain was different from all of published genes the bioassay showed that there was more infectivity with T4-3 or cyz-13 compated to standared strain, HD-1. |
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| Keywords: | Bacillus thuringiensis cry-type gene PCR-RFLP analysis bioassay |
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