农杆菌介导大豆遗传转化研究进展及其应用

农杆菌介导是大豆遗传转化的主要手段。近几年来大豆遗传转化方法得到了快速发展,但是仍然存在受体基因型匮乏、转化效率低,再生系统不完善等问题,为了进一步探讨大豆遗传转化的主要问题和策略,本研究归纳了近年来农杆菌介导大豆遗传转化在受体基因型及外植体选择,菌株筛选,标记基因选用和培养基组成等方面所做的改进,以得出有效的解决方法。并且介绍了农杆菌介导大豆遗传转化在作物改良和功能基因组学上的应用。

Research Advances and Application of Agrobacterium-mediated Transformation in Soybean

Genetic transformation of soybean (Glycine max (L.) Merr) depends mainly on Agrobacterium mediation system. In recent years, the soybean genetic transformation has been developed rapidly, but there are still some problems such as lack of genotypes, low transformation efficiency, imperfect regeneration system and so on. In order to explore the main problems and strategies of soybean genetic transformation, in this review, the author introduced the modifications of the technical procedures including the selection of genotypes and explants, strains screening, marker gene selection and medium composition, in order to obtain effective solutions. And the author also introduced the application of the Agrobacterium-mediated soybean transformation in crop improvement and functional genomics.