HPLC法检测建泽泻中23-乙酰泽泻醇B含量

〔目的〕建立中药材建泽泻中23-乙酰泽泻醇B的提取及检测体系。〔方法〕采用石油醚超声处理,辅助旋转蒸发提取23-乙酰泽泻醇B,利用高效液相色谱法检测;色谱柱:Waters C18柱(4.6 mm×250 mm,5μm);柱温:室温;流动相:乙腈-水(80∶20);流速:0.8 mL/min,检测波长:208 nm。〔结果〕建泽泻中23-乙酰泽泻醇B在25~500μg/mL的线性关系良好;平均回收率为98.1%,RSD为3.10%。〔结论〕该方法简便、准确、重复性好,适用于微量建泽泻的检测。

Determination of Alisol B 23-acetate in Rhizoma Alismatis by HPLC

〔Objective〕 To establish a method for extraction and determination of alisol B 23-acetate in Rhizoma Alismatis.〔Methods〕Extraction alisol B 23-acetate by following steps:ultrasonic extraction with petroleum ether,condensation of the extraction liquid by rotary evaporator,and determination by HPLC. Chromatographic column was Waters C18(4.6 mm×250 mm,5 μm),column temperature was room temperature,mobile phase was acetonitrile-water(80∶20),the rate flow was 0.8 mL/min,and the detection wavelength was 208 nm. 〔Results〕The calibration curves showed good linearity in the range of 25 μg/mL to 500 μg/mL of alisol B 23-acetate in Rhizoma Alismatis;the average recovery of alisol B 23-acetate was 98.1%,and the relative standard deviation was 3.10%. 〔Conclusion〕This method is reliable,simple and precise. It could be used for the detection of Rhizoma Alismatis.