快速检测大豆籽粒中十二种异黄酮组分的HPLC方法

 【目的】利用高效液相色谱（HPLC）技术快速检测大豆异黄酮组分，为大豆的异黄酮标记辅助育种提供重要依据。【方法】以大豆异黄酮12种组分为标准样品，利用HPLC技术结合吸收波峰鉴定的方法进行异黄酮组分的分析。【结果】不同异黄酮苷元组分黄豆苷元（daidzein）、大豆黄素(glycitein)和染料木素(genistein）分别具有不同的最大紫外光谱吸收波长，分别位于250、257和260 nm左右。采用YMC-C18反相色谱柱，以含0.1%（v/v）乙酸13%～30%（v/v）的乙腈为流动相，在35℃柱温条件下，经梯度洗脱，结合紫外吸收检测，可以准确地定性和定量鉴定出12种不同异黄酮组分。【结论】该方法样品用量少，检测异黄酮含量准确、快速，适合大量样本分析。

A Rapid HPLC Method for Determination of 12 Isoflavone Components in Soybean Seeds

【Objective】 It is highly important and has utilization values to determine the isoflavone components by HPLC as it can provide bases for molecular assistant selection of isoflavone in soybean breeding. 【Method】 Based on the standard samples of 12 isoflavone components, the isoflavone components were analyzed using the determination of absorbance peaks method by HPLC. 【Result】 The results showed that there were different maximum absorbance wavelengths for the daidzein, glycitein, and genistein, which were 250 nm, 257 nm and 260 nm, respectively. A linear gradient elution of acetonitrile (13%-30%) containing 0.1% acetic acid using a mobile phase was applied on a YMC-C18 column at 35 ℃. The 12 isoflavone components were determined using the UV detector by HPLC. 【Conclusion】 It is concluded that this is a rapid, precise method which adapted to determine the large numbers of samples with microanalysis.