| 应用RAPD技术分析绒山羊DNA的多态性 |
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| 引用本文： | 梁艳,刘迎春,张润吾,周欢敏.应用RAPD技术分析绒山羊DNA的多态性[J].内蒙古农业大学学报(自然科学版),2005,26(3):21-24. |
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| 作者姓名： | 梁艳 刘迎春 张润吾 周欢敏 |
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| 作者单位： | 内蒙古农业大学动物科学与医学学院,呼和浩特,010018;内蒙古库布齐沙漠研究所,达拉特旗,014300;内蒙古农业大学生物工程学院,呼和浩特,010018 |
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| 基金项目： | 内蒙古自治区教育厅重点领域研究项目 |
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| 摘 要： | 本实验应用RAPD技术,对辽宁多绒山羊、辽宁绒山羊、内蒙古阿尔巴斯白绒山羊和杂种多绒山羊的22个DNA模板进行了多态性比较和遗传距离分析,根据聚类分析构建了系统发生树状图.36条引物中筛选出11条引物具有多态性扩增片段.得到多态性位点59个,占总位点数的90.77%,分析显示杂种多绒山羊和辽宁绒山羊的亲缘关系较近.
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| 关 键 词： | 绒山羊 RAPD 遗传多样性 |
| 文章编号： | 1009-3575(2005)03-0021-04 |
| 收稿时间： | 2005-06-17 |
| 修稿时间： | 2005年6月17日 |
THE GENEFIC DSVERSITY ANALISIS OF CASHMEVE GOAFS DNA BY RAPD TECHNOLOGY |
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| LIANG Yan,LIU Ying-chun,ZHANG Run-wu,ZHOU Huan-min.THE GENEFIC DSVERSITY ANALISIS OF CASHMEVE GOAFS DNA BY RAPD TECHNOLOGY[J].Journal of Inner Mongolia Agricultural University(Natural Science Edition),2005,26(3):21-24. |
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| Authors: | LIANG Yan LIU Ying-chun ZHANG Run-wu ZHOU Huan-min |
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| Institution: | 1. College of Animal Science and Medicine,Inner Mongolia Agricultural University, Hohhot 010018, China; 2 Kubuqi Desert Institute,Dalate Banner,014300, China; 3 College of Biology Engineering,Inner Mongolia Argricultural University,Hohhot 010018 ,China |
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| Abstract: | Random amplified polymorphic DNA(RAPD) analysis was used to estimate genetic diversity and relationship in 22 DNA templates which belong to three Inner Monglia cashmere goat breeds and liaoning cashmere goat breeds.36 primers were used,11primers were screened to generate polymorphic bands.The genetic distance matrix was obtained by proportion of shared fragments.Pendrogram were generated by UPGMA.The results indicated that two Liaoning cashmere goats were clustered together.Kubuqi Cashmere goats was cldosely related to Liaoming cashmere. |
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| Keywords: | cashmere goats RAPD genetic diversity |
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