黄土高原芨芨草光合与蒸腾作用的初步研究

对芨芨草叶片的生理生态特性进行了初步分析,结果表明,1)芨芨草叶片光合速率日进程受环境影响,在不同时期差异较大,6、7月呈双峰曲线,8月和9月,光合速率则呈单峰曲线;芨芨草蒸腾速率和气孔导度日进程在整个生育期内基本上呈单峰曲线。2)回归分析和相关分析表明,不同时期对芨芨草叶片光合速率、蒸腾速率和气孔导度起决定性作用的环境因子不同,而在整个生育期它们三者之间都存在着极显著的正相关关系。     

不同盐浓度对芨芨草种子萌发过程中几种生理指标的影响

不同浓度的N aC l处理芨芨草种子后,通过研究其萌发过程中淀粉酶、超氧化物歧化酶、过氧化物酶活性以及可溶性糖含量的变化。结果表明:N aC l处理下芨芨草种子萌发过程中超氧化物歧化酶、过氧化物酶活性与对照相比均上升,且呈现双峰曲线的变化趋势。而淀粉酶活性、可溶性糖含量与对照相比均下降,并与N aC l处理浓度呈负相关。     

虎纹凤梨的组培快繁技术

用虎纹凤梨母株两侧的蘖芽经消毒处理后接种到MS＋6-BA 1.0 mg/L＋IAA 0.3 mg/L的培养基中,诱导出愈伤组织。而后用改良MS培养基＋6-BA 0.3 mg/L＋NAA 0.01 mg/L从愈伤中诱导出小芽并继代培养,扩繁。增殖率3.0～4.0倍。续代3～5代后从芽团上选取H＞2.5 cm的粗壮单株仍用改良MS培养基＋6-BA 0.3 mg/L＋NAA 0.01 mg/L扩繁,增殖倍数2～3倍。而芽团上H＜2.5 cm的丛芽分成具2～3个芽的小芽团用改良MS培养基＋6-BA 0.05 mg/L＋IAA 0.01 mg/L＋C 100 mg/L使其长高长粗壮,增殖倍数1.5倍,经过一段时间的培养后切取达到生根标准的单株接到1/4 MS＋IAA 0.3 mg/L＋NAA 0.05 mg/L＋C 500 mg/L培养基中,30～40 d生根率达95%以上。炼苗20 d左右移栽,成活率达85%～90%以上。     

Morphological responses of bedding plants to three greenhouse temperature regimes

Seedling crops of gazania, geranium, marigold, mimulus, pansy, verbena and vinca were grown to 60% anthesis in three greenhouse temperature regimes: (a) 26 °C day/6 ± 3 °C night (LT), with a 16.5 °C mean daily temperature (MDT), (b) 26 °C day/17 ± 4 °C night, with a 21 °C MDT, and (c) variable day/night temperatures in response to heating and cooling by a phase change material energy storage module (PCM) with an 8 ± 2 °C night and a 16 °C MDT. Crops bloomed from 2 to 17 days earlier in the 26 °C/17 °C greenhouse. The morphological characteristics of all cultivars of gazania, mimulus, pansy and verbena, geranium and vinca were similar regardless of greenhouse temperature regime. Marigold diploid and triploid cultivars and mimulus were morphologically more variable than the other bedding plant species. Crops grown in the PCM and LT greenhouses had virtually identical morphological characteristics and bloomed in about the same number of days. Plant height was not affected by DIF environments in the greenhouses. The PCM and LT greenhouses had a similar MDT, but the distribution of numbers of hours of mean daily temperatures was different.     

焚烧对芨芨草产量和品质的影响

本文研究了焚烧对芨芨草生产力的影响。焚烧后芨芨草在开花期可增产６２．２％，其营养价值在生长前期有所提高，以粗蛋白质在来分含量的提高和无氮浸出物与纤维素的降低为特征，烧后芨芨草期可延长１５天左右，叶量增大，地下部增重大于地下部。     

PEG处理对提高一串红种子活力的研究

本文以优，中，劣三个活力不等的一串红种子为材料，研究了PEG不同浓度和不同处理时间对种子发芽率的影响，结果表明，（1）PEG可明显改善中，低活力种子细胞膜透性，显著提高种子发芽率；（2）劣质一串红种子，用25%PEG处理1天或10%处理4天，其发芽率比对照提高16.12倍，19.2倍，中等活力一串红种子用10%PEG处理1天较佳；而优良种子经过处理，反而效果不好。     

两种矿山生态型香薷对铜的吸收、分布和积累特性研究

Two ecotypes of Elsholtzia, Elsholtzia splendens and E. argyi, are dominant plants growing on Cu and Pb-Zn smelters, respectively. Samples of the two ecotypes and the corresponding soils from fields of a copper mining area and a Pb-Zn mining area of Zhejiang Province, China, were analyzed to investigate Cu or Zn tolerance of these two ecotypes. Effects of nine Cu levels (0, 5, 10, 20, 40, 80, 160, 240 and 320 mg Cu L^-1 as CuSO₄·5H₂O) on growth and uptake, translocation and accumulation of Cu in these two ecotypes were examined in a solution culture experiment. The experimental results showed that dry weights (DW) of shoots and roots were depressed, and growth of E. splendens was less depressed than that of E. argyi when treated with ≥ 5 mg Cu L^-1. Concentrations of Cu in shoots of E. splendens and E. argyi exceeded 1 000 mg kg^-1 DW at ≥ 40 mg Cu L^-1. The maximum Cu accumulated in the shoots of Cu-treated E. splendens and E. argyi reached 101 and 142 μg plant^-1. Furthermore, analysis of plant samples from the fields showed that these two ecotypes can tolerant excess heavy metals and produced high dry matter, and E. splendens can accumulate 11.7 mg Cu plant^-1 grown on the Cu smelter. Therefore, E. splendens and E. argyi could be good plants for phytoremediation.     

多效唑对一串红生长和开花的影响

作者用不同浓度的多效唑(PP_(333))对一串红进行了叶面喷施试验。结果表明:PP_(333)能明显地抑制一串红的茎与叶的生长,使植株矮化,节间缩短;叶片变小,叶色加深;花序粗壮,花序数增加,花色艳丽,提高了观赏价值。应用PP_(333)对一串红叶面喷施时,500～1000ppm的浓度是适宜的。     

芨芨草基因组DNA提取与RAPD反应体系优化

本文选出适合芨芨草Achnatherum splendens (Trin.) Nevski基因组DNA提取方法,对RAPD反应体系中的一些重要参数进行优化,包括模板浓度、Mg2+、dNTP、引物和Taq酶浓度,建立了适合芨芨草RAPD分析的PCR反应体系.即在12.5uL的反应总体积中Mg2+浓度为1.5mmol·L-1,dNTPs 浓度0.2mmol·L-1,Taq酶0.5U,20ng模板DNA,10bp附机引物3.2pmol.反应程序为:前6个循环为95℃变性30s,35℃复性45s,72℃延伸80s,后36个循环为94℃ 45s,36℃ 40s,72℃ 70s,最后72℃延伸10 min.该反应体系具有良好的稳定性和重复性.     

一串红良种繁育技术的研究

应2008奥运会的需求,象征中国喜庆的红色花卉的繁育成为热点。本文提出一串红的良种繁育制度和种子生产技术规程,并以新品种的良种繁育为核心,开展了相关研究,以供良种的繁育。