Population means and correlation analyses of growth parameters in lisianthus (Eustoma grandiflorum Shinn.)

Summary The variation in growth characteristics among lisianthus (Eustoma grandiflorum Shinn.) genotypes was investigated. The genetic control of several growth parameters was studied by analysing parental, F1, BC1 and F2 populations. The parameters analysed were stem elongation rate (SE), stem diameter (SD), leaf width (LW), number of nodes (NN), node length (NL) and days to flowering (DF). The genotypes tested exhibited great variation for all parameters. A particularly wide variation was noted for SE, ranging from 3 to 56 mm/week. This variation was partially attributed to a differential sensitivity to rosette formation. There was a marked positive heterosis for SE, SD, LW and NN; these parameters proved to be genetically correlated within segregating populations. DF was slightly negatively correlated with SE, NN and NL. It was concluded that SE and DF were independently controlled.Contribution from the Agricultural Research Organization, The Volcani Center, Bet Dagan, Israel. No. 1196-E, 1993 series.     

Quantitative Genetic Analysis of Growth Rate in Lisianthus

A study was made of the inheritance of stem elongation rate during early (SER1) and late (SER2) vegetative growth period, of mean leaf width (MLW) and of days to flowering (DTP) in lisianthus (Eustoma grandiflorum Shinn.). The analysis was based on the data of an F1 diallel cross between different breeding lines. Marked directional heterosis was found for SER1, SER2 and MLW, but not for DTF, in which the dominance effects were incomplete and non-directional. SER1 and SER2 were positively correlated with MLW, but negatively correlated with DTP. The implications and the potential of heterotic growth rate of F1 hybrid lisianthus are discussed.     

农杆菌介导菜豆几丁质酶基因转化洋桔梗

通过农杆菌介导法将抗真菌的几丁质酶基因转入洋桔梗,以提高其对真菌病害的抗性。结果表明,成功克隆了抗真菌活性较强的菜豆几丁质酶基因,构建了植物表达载体,并对洋桔梗Double Mariachi Pink叶块进行转化,获得了卡那霉素抗性植株,对抗性植株进行了2次PCR检测(nptⅡ基因),第1次PCR检测获得了13个阳性株系,培养60 d后再进行第2次PCR检测,获得11个阳性株系,再对生长良好的5个转基因株系进行RT-PCR检测,获得了3个阳性株系。3个阳性株系的几丁质酶平均活性(0.215 U、0.225 U和0.286 U)均显著高于未转化植株(0.133 U),转基因株系几丁质酶粗提液对大豆链霉菌5A、5E和灰葡萄孢菌3种指示真菌的抑菌圈直径均显著大于未转化植株,其中抑菌效果最好的株系对上述3种指示真菌的抑菌圈直径与对照相比,分别增大了106.00%、90.91%和71.53%。     

Genetic transformation of Eustoma grandiflorum Griseb. by microprojectile bombardment

Summary Foreign DNA was introduced into cell suspension cultures and leaf tissue of Eustoma grandiflorum Griseb. (lisianthus) by microprojectile bombardment. For this purpose a low-cost bombardment device that uses a helium flux to accelerate microprojectiles was built. When cell suspensions were used, an average of 4.1 Kan resistant calli were recovered per shot after 4 months' cultivation on selective medium. Most of the Kan resistant plants regenerated from calli were positive to GUS assay. Both the nptII and gus genes were successfully amplified from alkali-treated leaves of putative transgenic plants by PCR analysis. Transgenic plants were not recovered from bombarded leaves. Considering the host range specificity of Agrobacterium, and the response of the species to plant regeneration from suspension culture, microprojectile bombardment is, at present, the most efficient procedure for genetic transformation of lisianthus.Abbreviations BA 6-benzyladenine - Cx cefotaxime - 2,4 D (2,4-dichlorophenoxy) acetic acid - FDA fluorescein diacetate - gus -glucuronidase - IAA indole-3-acetic acid - IBA indole-3-butyric acid - 2iP (2-isopentenyl) adenine - Kan kanamycin - nptII neomycin phosphotransferase II - PCR polymerase chain reaction