基于紫花苜蓿混播草地的全草型肉羊放牧育肥模式案例研究

本研究旨在探讨紫花苜蓿（Medicago sativa L.）混播草地放牧利用的生产潜力和集成全草型集约化肉羊放牧育肥技术模式。2017-2018年，在位于河北省廊坊市的中国农业科学院国际农业高新技术产业园内开展了2年羔羊育肥的划区轮牧试验，研究了紫花苜蓿混播草地的地上生物量、育肥羊增重以及羊肉品质的变化规律。结果表明：混播草地地上生物量超过11.2 tDM·hm^-2，5月份紫花苜蓿混播草地的粗蛋白为20.92%，每公顷紫花苜蓿混播草地可满足51只育肥羊、放牧育肥150天，每只增重30 kg所需的干物质、代谢能和粗蛋白三个方面的全部需求。与此同时，紫花苜蓿混播草地还可以显著增加羊肉中欧米伽3型多不饱和脂肪酸的含量，n-6/n-3比例为2.72，羊肉健康品质显著提高。以紫花苜蓿混播草地为基础的全草型肉羊放牧育肥技术具有广阔的应用前景，对我国苜蓿产业以及草地畜牧业发展具有重要意义。     

Death of a neonatal lamb due to Clostridium perfringens type B in New Zealand

ABSTRACT

Case history and clinical findings: A flock of 20 sheep was kept within three paddocks on a single property. None of the animals in the flock had been vaccinated against any disease for at least three years. Abdominal bloating and haemorrhagic diarrhoea were observed in Lamb 1 at 24 hours-of-age. The lamb subsequently died within an hour of the onset of clinical signs. Lamb 2 was 3-days-old when observed to be recumbent with opisthotonus. The lamb was treated with dextrose, vitamins B1 and B12, and penicillin G, but died 4 hours later.

Pathological findings: Examination of Lamb 1 revealed markedly increased gas within the peritoneum and within dilated loops of intestine. The intestines were dark red and contained large quantities of haemorrhagic fluid. Histology of the intestines revealed peracute mucosal necrosis with minimal accompanying inflammation. The intestinal lumen contained cell debris, haemorrhage, and myriad large Gram-positive bacilli. The intestines of Lamb 2 did not appear bloated or reddened. However, multiple fibrin clots were visible within the pericardial sac. Histopathological examination revealed small foci of necrosis within the mucosa of the distal intestine. The necrotic foci were often associated with large numbers of large Gram-positive bacilli.

Immunohistochemsitry and molecular biology: Intestinal samples from Lamb 1 were processed for Clostridium perfringens immunohistochemistry, which revealed large numbers of intralesional, positively immunostained rods. Fragments corresponding to the expected sizes for genes encoding alpha, beta, and epsilon C. perfringens typing toxins were amplified by PCR from DNA extracted from formalin-fixed sections of intestine.

Diagnosis: Lamb dysentery due to C. perfringens type B.

Clinical relevance: C. perfringens bacteria have a worldwide distribution, but disease due to C. perfringens type B has only been diagnosed in a small number of countries and has never been reported in New Zealand or Australia. C. perfringens type B produce both beta toxin and epsilon toxins, therefore both haemorrhagic enteritis and systemic vascular damage can develop. As many animals are exposed to C. perfringens without developing disease, there must be additional unknown factors that resulted in disease in these particular sheep. Vaccines that specifically protect against C. perfringens type B are available and may be recommended for use in smaller non-commercial flocks, as in the present case.     

外植体不同长度及接种方式对马尾松愈伤组织诱导的影响

为研究外植体不同长度及接种方式对马尾松愈伤组织诱导率的影响,将马尾松的外植体按照长度与接种方式的不同分别将其分组,比较组间差异。结果表明:外植体最佳接种长度为0.1~0.2 cm,愈伤组织细胞在切口处大量增殖和生长,外植体全部分化成愈伤组织,愈伤组织诱导率高达84.00%,几乎没有褐化和污染现象发生;外植体最佳接种方式为竖直接种,竖直接种的外植体愈伤组织形成较早,且褐化和污染程度较低。     

Adaptation of the LIGNUM model for simulations of growth and light response in Jack pine

LIGNUM is a whole tree model, developed for Pinus sylvestris in Finland, that combines tree metabolism with a realistic spatial distribution of morphological parts. We hypothesize that its general concepts, which include the pipe model, functional balance, yearly carbon budget, and a set of architectural growth rules, are applicable to all trees. Adaptation of the model to Pinus banksiana, a widespread species of economic importance in North America, is demonstrated.

Conversion of the model to Jack pine entailed finding new values for 16 physiological and morphological parameters, and three growth functions. Calibration of the LIGNUM Jack pine model for open grown trees up to 15 years of age was achieved by matching crown appearance and structural parameters (height, foliage biomass, aboveground biomass) with those of real trees. A sensitivity study indicated that uncertainty in the photosynthesis and respiration parameters will primarily cause changes to the net annual carbon gain, which can be corrected through calibration of the growth rate. The effect of a decrease in light level on height, biomass, total tree branch length, and productivity were simulated and compared with field data. Additional studies yielded insight into branch pruning, carbon allocation patterns, crown structure, and carbon stress. We discuss the value of the LIGNUM model as a tool for understanding tree growth and survival dynamics in natural and managed forests.     

影响马尾松种子园产量的种实害虫及综合防治

福建省漳平五一林场马尾松种子园球果常见的害虫有16种,隶属4目12科。其中微红梢斑螟、松实小卷蛾、油松球果小卷蛾和芽梢斑螟是影响良种产量的主要害虫。主要危害嫩梢、花芽、雌雄花、球果、种子等,使球果产量降低。采用营林措施和辅以“佳多频”振式杀虫灯进行防治,可有效地降低虫口密度,达到防治效果。     

尾巨桉、马占相思和马尾松人工林的土壤肥力比较

通过改进的内梅罗(Nemerow)综合指数对尾巨桉、马占相思、马尾松三种人工林的土壤肥力进行了综合评价,结果表明三种人工林A层土壤肥力综合指标值是尾巨桉>马占相思>马尾松,B层土壤肥力综合指标值是马占相思>马尾松>尾叶桉。综合分析表明尾巨桉林、马尾松林和马占相思林土壤肥力相差不大。     

马尾松人工林抚育间伐起始期的研究

通过对马尾松人工林试验地调查并选取标本木进行树干解析，分析马尾松人工林间伐合理起始期及不同起始期对林分生长影响，结果表明：初植密度高，间伐合理起始期提前，反之，间伐合理起始期推迟。超过间作合理起始期间伐会抑制林木生长。间伐合理起始期可以通过分析胸径加年生长量来确定。     

修枝对杉木幼林土壤肥力的影响初报

在福建洋口林场4年生的杉木人工林建立无节材培育试验地。选择修枝木,每年对其进行1次修枝,修枝强度为4种规定树干直径(6 cm、8 cm1、0 cm和12 cm)以下的所有枝条。试验3 a后研究不同修枝强度对7年生杉木林土壤肥力的影响,结果表明,试验后3 a,所有修枝试验林地的土壤0~10 cm和10~20 cm层次有机质、全N、水解性N和pH值的平均值均高于不修枝的对照,但差异未达到统计显著水平(P>0.05)。不同修枝强度及对照林地大多数土壤性质之间的差异未达到显著水平(P>0.05)。修枝后土壤性质有所改善,这可能与修枝后林下植被得到恢复有一定关系。修枝对杉木林地土壤性质的长期影响有待于今后进一步观测。     

马尾松共生菌根真菌调查研究

通过野外调查和室内鉴定,已查明与马尾松共生的菌根真菌27个种,其中有19个种是国内首次报道的。编制了马尾松菌根真菌名录(一)。马尾松隶属外生菌根树种,它可与多种菌根真菌共生形成典型的外生菌根的结构。     

三峡库区莲峡河小流域马尾松水文生态效应

2004年,在三峡库区莲峡河小流域马尾松林地进行了大气降水、林内透雨、树干茎流的测定,进行了林下枯落物和土壤对降雨影响的研究.结果表明:观测期,马尾松林内透雨占大气降水总量的76.70%,林冠层截留量占降雨总量的23.03%,树干茎流占降雨总量的0.27%;林下枯落物的最大持水量为3.40 mm,林地0～40 cm层土壤饱和蓄水量为183.60 mm;林内透雨、树干茎流、林冠截流与大气降水量以及降雨强度具有显著的二元线性相关关系.