以塘坝为中心建设立体型综合开发小区

以塘坝为中心建设立体型综合开发小区于明（黑龙江省青冈县水土保持办公室，１５１６０６）塘坝是我国水利水保建设的重要工程措施之一。它不仅可防洪蓄水，防治水土流失，同时对解决农田抗旱灌溉、养鱼等综合开发利用具有重要作用。搞好以塘坝建设为中心的小流域综合开发...     

搞好水土保持 造福子孙万代

搞好水土保持造福子孙万代肖作福（辽宁省人民政府，沈阳１１００３２）从５０年代初期，辽宁省开始了水土保持工作的征程，现已走过四十多年的历程。辽宁人民在辽宁大地上，奋斗不息，重新描绘着山河面貌，谱写了水土保持工作的新篇章。辽宁省的自然状况是“六山一水三分...     

水土保持是山丘区经济发展的根本出路

水土保持是山丘区经济发展的根本出路肖秧（四川省人民政府，成都６１００００）四川地处长江上游，幅员辽阔，境内高山峻岭，江河纵横，是一个有一亿多人口的农业大省。由于历史原因，森林曾遭严重破坏，加之人口众多，人均耕地数量少，垦殖指数高，水土流失十分严重。遥...     

新疆水土保持情况调查

新疆水土保持情况调查郭廷辅，佟伟力（水利部水土保持司，北京１００７６１）我们于１９９４年８月１１日至２６日对新疆伊犁、和田、喀什、吐鲁番、昌吉、乌鲁木齐等６个地区（州、市）的１１个县（市）、两个流域的水土流失情况和开展治理工作做了典型调查。同时，也对...     

小流域治理成果的开发利用途径

根据市场经济的要求和目前小流域治理中普遍存在的高投入、低效益问题，提出以市场经济为导向，通过合理开发利用治理成果，优化组合资源、资金、劳力、技术等生产要素，努力发展商品生产，提高小流域治理的经济效益。结合各地实践，总结出基本农田的集约经营、水保林的经营增值、水保草的养畜转化、非生产地的开发利用、转化产品的加工增值和疏通流通渠道六条开发利用途径。     

小麦品种对土传小麦黄色花叶病毒病抗性遗传的初步研究

本试验通过杂交、回交和抗病性鉴定等方法,测定了14个组合的F_1代对土传小麦黄色花叶病毒病的抗性遗传。根据双亲正反杂交表现型一致的结果,可以确定控制小麦品种对该病毒病的抗性为细胞核遗传。通过对F_1、F_2、BC_1及BC_2各代群体抗病株和感病株分离比值的分析,初步确定有关土传小麦黄色花叶病毒病的抗性遗传可能受两对致病显性基因(S_1、S_2),和一对抑制基因(I)所控制,作者并对这一问题与前人工作作了比较和讨论。     

Epidemiology of beet necrotic yellow vein virus in sugar beet at different initial inoculum levels in the presence or absence of irrigation

A field experiment was set up in 1988 to study the development of rhizomania disease of sugar beet at different inoculum levels of beet necrotic yellow vein virus (BNYVV) in soil. Five, tenfold different, inoculum levels were created by addition of the approximate amounts of 0, 0.5, 5, 50 and 500 kg infested soil per ha (the latter corresponding to 0.01% v/v calculated to the tillage layer). A drip irrigation treatment was applied to study the influence of soil moisture on disease. Susceptible sugar beet, cv. Regina, was grown for three consecutive years.In the first year, root symptoms were not observed, but BNYVV-infected plants were detected by ELISA in low numbers at all inoculum levels at harvest. After late drilling in 1989, high numbers of infected plants, up to 90–100% in plots with the highest inoculum level, were detected already in June. Root symptoms were also observed from June onwards. In both these years disease incidence increased in time and was significantly influenced by the initial inoculum level. In the third year, the whole field was heavily diseased, and only for the non-irrigated plots incidence differed for different initial inoculum levels. The expression of symptoms by BNYVV-infected plants was influenced by initial inoculum level, thus by the amount and timing of primary infection.Root weight at harvest was not affected, but sugar content decreased with increasing inoculum level already in 1988, leading to a reduction in sugar yield of 10% at the highest inoculum level. In 1989, both root weight and sugar content decreased progressively with increasing inoculum level, resulting in sugar yield reductions of 11–66% (down to approximately 3000 kg ha^–1) for low to high inoculum levels compared to the control. As the control plots became contaminated, all yields were low in 1990, still showing a decrease with increasing inoculum level in the non-irrigated plots, but an overall mean sugar yield of 3323 kg ha^–1 for the irrigated ones.Sodium and -amino nitrogen content of the root, additional quality parameters determining extractability of sucrose, showed an increase and decrease, respectively, with increasing initial inoculum level already in the first year. The relative differences in contents compared to those from the control were largest for Na content. A significant negative correlation was found between Na (mmol kg^–1 root) and sugar content (% of fresh weight); linear for 1988, exponential for 1989 and 1990.In spring 1989, the infestation of individual plots was assessed using a quantitative bioassay estimating most probable numbers (MPNs) of infective units of BNYVV per 100 g dry soil. The relationship between the MPns determined and root weight, sugar content and sugar yield at harvest could be described by Gompertz curves. The increase in disease incidence with increasing MPN in 1989 was adequately fitted with a logistic equation.     

Role of miR-219 and TGFBR2 in pathogenesis of renal fibrosis

AIM:To study the role of microRNA-219 (miR-219) in regulation of transforming growth factor-β receptor type 2 (TGFBR2) in renal fibrosis. METHODS:The renal fibrosis patients (n=70) were selected in this stu-dy, and 20 cases of healthy people were selected as control group. RT-qPCR was used to detect the expression of miR-219 in the serum of the patients with renal fibrosis and control group, and the expression of miR-219 in NRK49F cells after stimulation with angiotensin Ⅱ(AngⅡ) was detected. The protein expression of α-smooth muscle actin (α-SMA) in the NRK49F cells transfected with miR-219 mimics after stimulation with AngⅡ was determined by Western blot. The potential target gene TGFBR2 of miR-219 was screened and verified by the method of luciferase reporter gene. RT-qPCR and Western blot were used to detected the effect of miR-219 mimics on the expression of TGFBR2 at mRNA and protein levels, and the mRNA expression of α-SMA, connective tissue growth factor (CTGF), type I collagen α1 (COL1A1) and COL3A1 in the NRK49F cells was also detected, respectively. The unilateral ureteral occlusion (UUO) mouse model was established and the expression of miR-219 in the renal tissue was monitored. The morphological change of renal fibrosis was observed in the UUO mice after injection of miR-219, and the mRNA expression levels of COL1A1 and COL3A1 were detected. RESULTS:The expression level of miR-219 in the patients with renal fibrosis was significantly lower than that in control group, and the expression of miR-219 in the UUO mice was decreased significantly (P<0.01). The expression level of miR-219 was significantly decreased in the NRK49F cells after AngⅡ stimulation, and miR-219 mimics inhibited the protein expression of α-SMA(P<0.01). miR-219 mimics had a targeted regulatory effect on TGFBR2 gene, which inhibited the mRNA and protein expression of TGFBR2. miR-219 mimics inhibited the mRNA expression of α-SMA, CTGF, COL1A1 and COL3A1. miR-219 also down-regulated the mRNA expression of COL1A1 and COL3A1 in the UUO mice and inhibited the process of renal fibrosis. CONCLUSION:miR-219 inhibits the development of renal fibrosis by inhibiting the expression of TGFBR2, which may become a new target for the diagnosis and treatment of renal fibrosis.     

电磁式电流互感器误差特性的分析

通过对电磁式电流互感器误差特性的分析，提出了互感器在正常工作时应注意的问题，进而提高互感器测量和保护的可靠性。     

英语双及物结构的构式语法观

构式语法是以认知语言学为理论背景的语言研究方法。本文以Goldberg所倡导的构式语法理论为指导，对双及物结构予以剖析，旨在探究双及物结构这一特定构式的句法和语义特征，同时构建出双及物结构的构式表征模式，以揭示该构式的本质。