Necrotic enteritis challenge regulates peroxisome proliferator-1 activated receptors signaling and β-oxidation pathways in broiler chickens

Necrotic enteritis (NE) is an important enteric disease in poultry and has become a major concern in poultry production in the post-antibiotic era. The infection with NE can damage the intestinal mucosa of the birds leading to impaired health and, thus, productivity. To gain a better understanding of how NE impacts the gut function of infected broilers, global mRNA sequencing (RNA-seq) was performed in the jejunum tissue of NE challenged and non-challenged broilers to identify the pathways and genes affected by this disease. Briefly, to induce NE, birds in the challenge group were inoculated with 1 mL of Eimeria species on day 9 followed by 1 mL of approximately 10⁸ CFU/mL of a NetB producing Clostridium perfringens on days 14 and 15. On day 16, 2 birds in each treatment were randomly selected and euthanized and the whole intestinal tract was evaluated for lesion scores. Duodenum tissue samples from one of the euthanized birds of each replicate (n = 4) was used for histology, and the jejunum tissue for RNA extraction. RNA-seq analysis was performed with an Illumina RNA HiSeq 2000 sequencer. The differentially expressed genes (DEG) were identified and functional analysis was performed in DAVID to find protein–protein interactions (PPI). At a false discovery rate threshold <0.05, a total of 377 DEG (207 upregulated and 170 downregulated) DEG were identified. Pathway enrichment analysis revealed that DEG were considerably enriched in peroxisome proliferator-activated receptors (PPAR) signaling (P < 0.01) and β-oxidation pathways (P < 0.05). The DEG were mostly related to fatty acid metabolism and degradation (cluster of differentiation 36 [CD36], acyl-CoA synthetase bubblegum family member-1 [ACSBG1], fatty acid-binding protein-1 and -2 [FABP1] and [FABP2]; and acyl-coenzyme A synthetase-1 [ACSL1]), bile acid production and transportation (acyl-CoA oxidase-2 [ACOX2], apical sodium–bile acid transporter [ASBT]) and essential genes in the immune system (interferon-, [IFN-γ], LCK proto-oncogene, Src family tyrosine kinase [LCK], zeta chain of T cell receptor associated protein kinase 70 kDa [ZAP70], and aconitate decarboxylase 1 [ACOD1]). Our data revealed that pathways related to fatty acid digestion were significantly compromised which thereby could have affected metabolic and immune responses in NE infected birds.     

陕西苹果产业SWOT分析及发展策略

在分析陕西苹果产业的优势、劣势、机会、挑战的基础上,提出扩张发展型、竞争型、防御型、保守型4种战略方案。     

Genetic variations in survival of rohu carp (Labeo rohita, Hamilton) after Aeromonas hydrophila infection in challenge tests

A challenge test against Aeromonas hydrophila was conducted using 2062 rohu carp (Labeo rohita) fingerlings obtained from 52 dams and 87 sires (87 full-sib families) of two year-classes (2003 and 2004). Attempts to establish a cohabitant challenge model were not successful. Therefore, fish were challenged by intraperitoneal injection with A. hydrophila in two replicate tanks per year-class, and dead fish were collected hourly. The mortality reached its peak at 16–22 h after challenge and had almost completely stopped after 58 h. The test was terminated after 382 h at which the average survival was 43.9 and 48.9% in the two 2003 year-class tanks, and 77.1 and 35.8% in the two 2004 year-class tanks. Heritability estimates for survival were obtained from sire and dam threshold models (THR) and sire and dam linear repeatability models (LINR). For both types of models the heritability estimates based on data from single tanks were not consistent. For both year-classes, data from one of the two challenged tanks demonstrated significant additive genetic variation in survival during the A. hydrophila infection, whereas the heritability estimates were not significantly different from zero for the other tank. Further, genetic correlation between survivals in the two replicate tanks in each year-class was not significantly different from zero. The differential results from the replicate tanks demonstrate that additional challenge test experiments are needed before firm conclusions can be drawn about the magnitude of additive genetic variation for survival to aeromonasis in rohu carp. A cohabitant challenge model that allows the testing of important defence mechanisms in the skin and mucous membranes of the fish might have been more appropriate. To establish a valid cohabitant challenge model for rohu carp should be given high priority.     

Optimization of experimental infection protocols for the estimation of genetic parameters of resistance to White Spot Syndrome Virus (WSSV) in Penaeus (Litopenaeus) vannamei

Two infection protocols, individual oral (IO) and waterborne infection (WB), were evaluated to challenge Penaeus (Litopenaeus) vannamei with White Spot Syndrome Virus (WSSV). Five different batches of full and half-sib families were infected experimentally and tested for growth performance under commercial growth conditions. The genetic variance for WSSV resistance was estimated using a linear sire-dam repeatability model that considers test-day survival as the dependent variable. The heritability estimates using the IO protocol ranged from 0.01 ± 0.00 to 0.02 ± 0.01, whereas the heritability estimates using the WB infection protocol were not significantly different from zero. The genetic correlations measured as the product moment correlation between full-sib family breeding values for resistance to WSSV and harvest body weight in ponds were unfavourable in three of the five batches and favourable in one of them. The dosage of WSSV was better controlled with IO oral infection than with other methods, with all animals being exposed to approximately the same risk of infection at the same time. This should improve the accuracy of the genetic parameters and hence improve the accuracy of the breeding values. It should, however, be noted that once the outbreak was established and the mortalities began, the shape and the magnitude of the slope of the mortality curves showed little difference in the infection pattern between batches irrespective of the dosage and infection protocol, and in most of the cases the cumulative mortality was greater than 80%. The main reason for this is probably the high densities of animals in the tanks needed for the genetic evaluations.     

Pathogenicity of white spot syndrome virus on postlarvae and juveniles of Penaeus (Litopenaeus) vannamei

White Spot Syndrome Virus (WSSV) has decimated the shrimp aquaculture around the world. Breeding efforts to generate resistant stocks are necessary but there is a lack of basic information on challenge test strategies focused on genetic selection. Infection routes and developmental stages were evaluated on Penaeus vannamei as a first step in a strategy to select white spot virus (WSSV)-resistant stocks. Mortalities could not be induced before the PL30 stage. The impact of infection by immersion and blended tissue was intermediate on mortalities when compared to the minced tissue treatment on PL30. Blended and minced tissue treatments produced the highest mortalities on PL40 while immersion was intermediate. A general tendency towards higher susceptibility associated with older stages was detected. Additionally, juveniles of 1 g average weight from three local breeding programs were challenged. There were no differences in survival between the programs, although two of them derived their progenies from survivors of strong WSSV events. The implications of these results to the WSSV epidemiological characteristics and breeding programs are discussed.     

十堰市蔬菜产业现状及对策

十堰市蔬菜产业在逆境中稳定发展,产品总量逐年增加,经济效益不断提升,基地类型趋于优化,科技应用逐步深入,市场流通相对完善,龙头企业稳步壮大,同时也面临着生产成本高、交易成本高、生产风险大、加工损耗大等挑战,且供求矛盾和价格波动长期存在。对十堰市蔬菜产业建设稳定的现状及主要问题进行剖析,系统性地提出了十堰市蔬菜产业发展对策,即明确责任目标,完善规划布局,强化政策支撑,保增基地面积,完善服务网络,做强龙头企业,整合品牌资源,完善建管机制。     

Onset of immunity in kittens after vaccination with a non-adjuvanted vaccine against feline panleucopenia, feline calicivirus and feline herpesvirus

The induction of a quick onset of immunity against feline parvovirus (FPV), feline herpesvirus (FHV) and feline calicivirus (FCV) is critical both in young kittens after the decline of maternal antibodies and in cats at high risk of exposure. The onset of immunity for the core components was evaluated in 8–9 week old specific pathogen free kittens by challenge 1 week after vaccination with a combined modified live (FPV, FHV) and inactivated (FCV) vaccine. The protection obtained 1 week after vaccination was compared to that obtained when the challenge was performed 3–4 weeks after vaccination. The protocol consisted of a single injection for vaccination against FPV and two injections 4 weeks apart for FHV and FCV.At 1 week after vaccination, the kittens showed no FPV-induced clinical signs or leukopenia following challenge, and after FCV and FHV challenges the clinical score was significantly lower in vaccinated animals than in controls. Interestingly, the relative efficacy of the vaccination was comparable whether the animals were challenged 1 week or 3–4 weeks after vaccination, indicating that the onset of protection occurred within 7 days of vaccination. Following the 1-week challenge, excretion of FPV, FHV and FCV was significantly reduced in vaccinated cats compared to control kittens, confirming the onset of immunity within 7 days of vaccination.     

入世后我国甜樱桃面临的机遇与挑战及发展对策

甜樱桃作为一种早熟、优质、保健果品,而且种植效益较高,在国内国际都正处于发展时期。根据世界甜樱桃生产和贸易情况,指出了我国在甜樱桃科学技术与生产上与世界先进甜樱桃生产国的差距,分析了我国加入WTO之后,我国甜樱桃面临的机遇和挑战,并且机遇大于挑战。如果有适当的科技投入,我国甜樱桃产业将可克服现在科技滞后的状态,发挥我国劳动力低廉和国土辽阔的优势,迅速扩大其生产总量,提高品质,扩大出口,增加农民的收入,2015年可望形成32.4～43.2亿元的社会总产值。     

“农超对接”深入开展所面临挑战及对策措施

对"农超对接"开展过程中构建阶段、运行阶段和实施保障等方面遇到的问题进行分析总结,并在此基础上提出了针对性的改进措施,即强化政府指导作用、完善相应制度、增强农民合作组织竞争力等。     

统筹城乡户籍制度改革与农村人口空心化：挑战与应对

从统筹城乡户籍制度改革对农村资源配置、农村有效投入、农村规模发展及农村技术人才4个方面分析统筹城乡户籍制度改革可能给农村发展造成人口空心化带来的影响和挑战,最后,基于土地流转、农村新型养老保险和发展农业机械化等角度提出了相应的建议。