经济林良种推广现状及政策建议

经济林良种是经济林产业的“芯片”,是经济林生产优质、高产、高效的物质基础。完善经济林良种推广政策,是实现经济林产业高质量发展的根本保障。通过数据收集和整理,系统梳理全国经济林良种推广现状,指出经济林良种推广中还存在着良种使用率不高,经济林低产低效;部分经济林良种存在超范围推广;良种审定过多,结构性过剩和种植品种过多,对接生产加工不畅等突出问题。进而提出从以种植为目标的良种推广转变为以生产加工等市场需求为导向的良种推广思路。从优化良种供给、加强良种推广的宏观指导、强化科技支撑和宣传培训、实施经济林提质增效工程和充分发挥产业协会、创新联盟的作用等方面给出优化经济林良种推广的政策建议。     

新鲜蔬菜硝酸盐含量测定的改进试粉法

 为适应新鲜蔬菜硝酸盐快速检测的需要, 在现有试粉法的基础上对硝酸盐测定的试粉法进行了改良研究。结果表明, 本研究建立的直接以去离子水浸提蔬菜匀浆, 混合试粉配方为柠檬酸∶一水硫酸锰∶无水对氨基苯磺酸∶N - 1 - 萘乙二胺盐酸盐∶细锌粉= 30∶4∶1.6∶0.8∶1, 其加入量为0.1 g的改进试粉法, 对于溶液中硝酸盐含量在0～20 mg/L范围时, 显色吸光值与硝酸盐含量呈现良好的线性关系, 相关系数达0.9999, 方法回收率在97.7%～104.5%之间, 相对标准偏差2.71%。用本改进试粉法测定11种蔬菜的硝酸盐含量与国标法测定结果的t检验具有一致性。     

改良热硼酸法高效提取苹果果实RNA

苹果果实、尤其是成熟期的果实中多糖和其他次生物质含量高,难以提取RNA。对此,在原热硼酸法的基础上进行了改进,通过添加提取辅助剂并根据辅助剂特性调整提取步骤,高效、稳定地从苹果果实、尤其是后期果实中提取到了高质量的RNA。所得RNA的A260/A230大于2.0,A260/A280为1.8-2.1,并且RNA产量高,其中前期果实RNA产率在13.40μg/g以上,后期果实RNA产率在7.02μg/g以上,完全可以满足RT-PCR、cDNA-AFLP等分子生物学实验的要求。     

Adopters,testers or pseudo-adopters? Dynamics of the use of improved tree fallows by farmers in western Kenya

Although there has been increasing research on the adoption of agroforestry technologies over the last decade, few such studies have assessed uptake over a long period and many are based on a single snapshot in time. Furthermore, most of these studies have mainly looked at non-adopters and adopters: only recently have social scientists considered testers. A further category of users neglected in adoption studies has been re-adopters of technologies. Studying this group provides an interesting and more nuanced understanding of adoption and re-adoption. Methodologically, most adoption studies use quantitative methods and fail to link their findings to wider socio-economic, political and institutional settings. This paper presents a study of the dynamics of improved tree fallow use by farmers in Siaya and Vihiga districts of western Kenya over a period of eight years. It uses both qualitative and quantitative data to critically discuss the motivations of adopters, testers/rejecters and re-adopters. The results show that the process of adoption is highly dynamic and variable with farmers planting improved fallows and discontinuing or re-adopting them due to a whole range of factors, of which soil fertility improvement is just one. These factors included incentives from projects, the tying of adoption to credit programmes, prestige, participation in seminars/tours and the availability of a seed market from projects promoting improved fallows. Farmers planting improved fallows for such reasons may be termed ‘pseudo-adopters’. There were significant differences in adoption between the two districts, with more farmers in Siaya planting improved fallows than in Vihiga. A majority of farmers in Vihiga (53%) who were given seed never planted improved fallows, even though they had been exposed to the technology. Some 40% of farmers in Siaya and 38% in Vihiga planted improved fallows but later rejected them. This has some important implications for research and development. For improved fallow technologies to be attractive to farmers, they must provide other tangible economic benefits besides soil fertility improvement. This presents a challenge to researchers who must better attune themselves to the needs and demands of farmers if they wish to see their research findings widely adopted.     

国家奶牛良种补贴项目在泾阳县实施的成效与启示

为今后奶牛改良工作成果的巩固和推广提供依据,对国家奶牛良种补贴项目在泾阳实施的效果与措施进行总结.结果表明:累计使用良种冷冻精液细管23.7万支,生产改良奶牛3.15万头,且已有1.1万头的优质母牛已正式进入产奶阶段.良种补贴后代奶牛的头胎日产奶量达到27 kg左右,比同期母亲日产奶量高2 kg～3 kg;生奶平均乳...     

Effect of dietary betaine levels on growth performance and hepatic intermediary metabolism of GIFT strain of Nile tilapia Oreochromis niloticus reared in freshwater

An 8‐week feeding experiment was conducted to determine the effect of dietary betaine levels on the growth performance and hepatic intermediary metabolism of genetically improved farmed tilapia (GIFT) strain of Nile tilapia Oreochromis niloticus (mean initial body weight: 78.3 ± 1.3 g, means ± SD). Six practical diets were formulated with the incorporation of betaine at the levels of 0 (control), 5, 10, 15, 20 and 25 g kg⁻¹. Survival showed no significant differences among the treatments (P > 0.05). The highest and lowest weight gain (WG) and specific growth rate (SGR) were observed for fish fed the diets containing 5 and 0 g kg⁻¹ (control) betaine, respectively. Feed intake showed similar trend with WG and SGR. In contrast, feed conversion ratio was the lowest when dietary betaine level was 5 g kg⁻¹. In general, dietary betaine supplementation showed no significant effect on hepatic composition of tilapia. Condition factor and viscerosomatic index tended to increase with increasing dietary betaine levels from 0 to 5 g kg⁻¹ and then decline when dietary betaine levels further increased from 5 to 25 g kg⁻¹. In contrast, hepatosomatic index declined with increasing dietary betaine levels (P < 0.05). Dietary betaine levels significantly influenced several hepatic enzymatic activities, including succinate dehydrogenase, lactate dehydrogenase, malic dehydrogenase, lipoprotein lipase and hepatic lipase, suggesting that dietary betaine addition had significant effects on nutrient metabolism in the liver. Based on the second‐order polynomial regression analysis of WG, 12.5 g kg⁻¹ of dietary betaine level seemed optimal for genetically improved farmed tilapia strain of O. niloticus.     

不同饲养管理条件下黄牛的同期发情试验

[目的]为了便于集中配种或胚胎移植。[方法]采取了三种方法：一次PG改进法、CI-DR＋PMSG＋PG＋LHRH-A3法、PMSG＋PG＋LHRH-A3法处理三种不同饲养管理条件下的黄牛,诱导同期发情。[结果]表明四次处理的同期发情率分别为100%、100%、98.8%、82.4%,人工授精或胚胎移植受胎率分别为27.3%、80%、76.5%、59.2%。[结论]从经济角度表明一次PG改进法、PMSG＋PG＋LHRH-A3法是比较切合实际,也比较理想的同期发情方法,效果也比较理想。     

Improved protocol for Agrobacterium mediated transformation of tomato and production of transgenic plants containing carotenoid biosynthetic gene CsZCD

Improved protocol for Agrobacterium mediated transformation of tomato (Lycopersicon esculentum) Micro-Tom was developed to use in corporation of the carotenoid biosynthetic genes CsZCD (Crocus zeaxanthin 7,8-cleavage dioxygenase). From these experiment, a transformation methodology using explants from cotyledons cultured for 1 day on the medium with zeatin 2 mg/L, IAA 0.1 mg/L, carefully submerged in the Agrobacterium inoculum for 20 min, then concultured with the agrobacterium for 3 days on the same medium, followed by a transfer to the same medium with 500 mg/L cefotaxin for 3 days and then by a transfer to the same medium with 100 mg/L kanamycin and 500 mg/L carabenillin for 6–8 weeks and resulted in a greater than 20% transformation efficiency in the concentration of Agrobacterium OD600 = 0.2 tested. In this transformation method, no feeder layers were used and the subculture media was minimal. Among the Agrobacterium concentrations of OD600 = 0.2, 0.5 and 1.0, the best transformation efficiency, 20.87%, was obtained by using OD600 = 0.2, which was significantly higher than that of OD600 = 1.0. The presence of the inserted target genes was checked using a rapid and efficient PCR test. The protocol was successfully employed in the production of transgenic Micro-Tom tomato containing the carotenoid biosynthesis CsZCD under constructive promoter. This procedure represents a simple, efficient and general means of transforming tomato.     

推土机履带行走系统的设计及工艺改进

针对T90/T100推土机履带行走系统存在拖带轮偏磨问题,分析其产生的主要原因是行车架焊合件加工质量不稳定,提出了控制质量的具体措施,取得了良好效果,为今后一系列推土机履带行走系统的设计改进提供了借鉴经验。     

Improvement of quality and shelf-life of strawberries with edible coatings enriched with chitosan

Strawberry fruit have a very short shelf-life and senescent period due to their high degree of perishability and infection caused by several pathogens that can rapidly reduce fruit quality. The aim of this study was to determine the efficacy of carboxymethyl cellulose (CMC), hydroxypropylmethyl cellulose (HPMC) and composites with chitosan (CH) coatings on the shelf-life and overall quality of strawberry fruit. Strawberry fruit dipped for 2 min in solutions of CMC (1%), HPMC (1%), CMC (1%) + CH (1%) and HPMC (1%) + CH (1%) were stored at 11 ± 1 °C, 70–75% RH, while the uncoated fruit served as controls. Fruit coated with edible coatings showed significant delays in the change of weight loss, decay percentage, titratable acidity (TA), pH, total soluble solids (TSS) and ascorbic acid content as compared to uncoated control fruit. In addition, the edible coatings had a positive effect on maintaining higher concentrations of total phenolics and total anthocyanins, which decreased in control fruit due to over-ripening and senescence processes. Compared to the controls, all the coatings had positive effects on the inhibition of cell wall degrading enzymes and among all the tested coatings, CMC + CH and HPMC + CH was superior in inhibiting enzyme activity. These findings suggest that the use of CMC 1% + CH 1% and HPMC 1% + CH 1% coatings are useful for extending the shelf-life and maintaing quality of strawberry fruit.