凤尾桑及其芽变品系间遗传变异的ISSR分析

利用ISSR引物,从分子水平分析了凤尾桑及其芽变株系的遗传变异。从8个ISSR引物扩增结果来看,3个引物的扩增产物在株系间无差异,从变异株系扩增条带来看,凤尾芽变变异最大。本结果从分子水平证明了芽变株系的遗传结构差异。     

SSR和ISSR标记及其在牧草遗传与育种研究中的应用前景

SSR和ISSR的高突变率、丰富的多态性以及在基因组中的广泛性,使其成为了居群遗传学研究、种质资源鉴定、亲缘关系分析、遗传图谱构建和分子标记辅助选择的重要手段.概述了SSR和ISSR在植物基因组中的分布、结构和遗传方式,并对其研究方法和应用前景进行扼要阐述,旨在为从事牧草遗传育种研究的工作者提供有益的帮助.     

凤凰单丛茶树资源遗传多样性的ISSR分析

采用ISSR分子标记技术对48份凤凰单丛茶品种(系)进行了遗传多样性分析。选用10个多态性高、分辨力强的ISSR引物分别对供试材料基因组DNA进行扩增,共获得121个位点,其中多态位点带98个,多态位点比率(PPB)为81.0%。品种(系)之间的遗传相似系数变化范围在0.590 9~0.967 4之间。UPGMA聚类分析结果表明,在GS值为0.792的水平上供试材料可划分为7大类,其亲缘关系远近与地理来源关系不大且与香味类型没有必然的联系。     

ISSR Analysis of M_1 Generation of Gladiolus hybridus Hort Treated by EMS

Gladiolus hybridus Hort is one of famous cutting flowers,being famous of big size,bright color,various shapes and long bloom period.New species should be cultivated in order to meet consumers' needs.Mutagenic breeding is a shortcut to cultivate new species of flowers.In this study,corm bud of G.hybridus Hort was treated with different concentrations of EMS.Then M 1 generation was analyzed by ISSR.Results showed that EMS was a very effective mutagenic agent for the corm bud of G.hybridus Hort.With the increa...     

橡胶树抗寒种质遗传多样性分析

应用RAPD和ISSR技术对20份橡胶树抗寒种质进行遗传多样性分析。结果表明，16个RAPD引物和12个ISSR引物分别扩增出条带113和101条，多态性条带指数(PPB)分别为61.1%和63.4%。据Nei-Li相似系数，ISSR标记在相似系数约0.74处，可将20份材料分为野生种质和栽培种质两大类；RAPD标记在相似系数约0.70处，也可将供试材料分为野生种质和栽培种质两类，但只有XJ002420为野生种质。对2种标记的分析结果进行相关分析，结果表明，RAPD和ISSR所检测的遗传相似系数呈极显著正相关，相关系数为0.672。以上结论表明，RAPD和ISSR标记可用于橡胶树种质资源的遗传多样性研究，但鉴于ISSR较RAPD有更强的多态性检出能力，ISSR技术应作为遗传多样性研究的首选单引物标记。     

贵州野生枇杷资源遗传多样性的ISSR分析

本文采用ISSR标记，构建了贵州39份野生枇杷种质的DNA指纹图谱，并对其遗传多样性进行了评价。结果表明，以筛选出的15条引物进行PCR扩增，获得清晰、重复性好的谱带125条，其中多态性谱带108条，多态性比例为86.4%；应用引物815、825和841的组合，构建了39份枇杷种质的ISSR指纹图谱，可以有效区分所有供试材料；15条引物的谱带数据聚类分析表明，供试种质的遗传相似性系数为0.40~0.98，在相似性系数为0.70时，可将供试种质分成五大类。聚类结果与种质表型相关，与地理分布的相关性不明显。     

Genetic diversity of Pueraria lobata (kudzu) and closely related taxa as revealed by inter-simple sequence repeat analysis

Kudzu (Pueraria lobata) is a noxious weed infesting some areas of the USA. Knowledge of its genetic variation in both native and invasive areas can lead to effective biological control measures. Inter-simple sequence repeat (ISSR) variations were studied in P. lobata and its four closely related congeneric species (P. edulis, P. montana, P. phaseoloides and P. thomsoni). ISSR results allowed a clear separation of these five species. For P. lobata, 108 plants from China and USA were analysed. The samples from the US were genetically closer to Chinese P. lobata populations than to other congeneric populations. High genetic differentiation was found for P. lobata, P. montana and P. thomsoni in Chinese samples. High genetic diversity and low population differentiation was found in P. lobata samples of the US. This supports the hypothesis of multiple introductions into the USA from different sources in Japan or China, followed by subsequent gene exchange and recombination.     

莲雾种质资源遗传多样性的ISSR分析

 对12份莲雾资源和2个莲雾近缘种进行了ISSR分析, 18条引物共扩增出459个位点, 其中多态性位点435个(94.77% ) 。利用UPGMA聚类分析表明: 蒲桃、马六甲蒲桃与莲雾分属于3个不同的组; 12个莲雾品种和品系又分成4个亚组, 这与传统分类学上按照果实成熟期的划分结果基本一致。     

Interspecific relationships of Lycoris (Amaryllidaceae) inferred from inter-simple sequence repeat data

Inter-simple sequence repeat (ISSR) markers were used to evaluate interspecific relationships of Lycoris. Twenty-four samples were included in the study representing a total of 20 among species and varieties. Thirteen primers produced 228 discernible DNA fragments, 205, or 89.91%, of which were polymorphic, indicating a high level of interspecific genetic variation in Lycoris. Our UPGMA cluster analysis recognized four major groups of species, which were consistent with morphological and karyotype observations. The first group included species with telocentric and metacentric chromosomes, while the second consisted of species with a haploid genome of 11 subtelocentric chromosomes. The third group contained species with a mixture of subtelocentric, telocentric and metacentric chromosomes. The last group included the Japanese and Korean species. Lycoris anhuiensis was clustered within accessions of Lycoris longituba, suggesting that it could be recognized as a variety of L. longituba. Our ISSR data also suggested that L. straminea may be a hybrid between Lycoris chinensis and Lycoris radiata var. pumila, and that L. caldwellii, an allotriploid, may be of a hybrid origin of L. chinensis and L. sprengeri.