| 凤凰单丛茶树资源遗传多样性的ISSR分析 |
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| 引用本文： | 吴清韩,庄东红,朱慧,赵庆芳,杨培奎,马瑞君.凤凰单丛茶树资源遗传多样性的ISSR分析[J].热带作物学报,2015,36(3):499-503. |
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| 作者姓名： | 吴清韩 庄东红 朱慧 赵庆芳 杨培奎 马瑞君 |
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| 作者单位： | 韩山师范学院生物学系;韩山师范学院生物学系;西北师范大学生物科学学院;韩山师范学院生物学系;韩山师范学院生物学系;韩山师范学院生物学系 |
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| 基金项目： | 广东省科技计划项目（No. 2011B020304012）；广东省高等学校科技创新重点项目（No. cxzd1131）；韩山师范学院博士启动项目（No. QD20140325）。 |
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| 摘 要： | 采用ISSR分子标记技术对48份凤凰单丛茶品种(系)进行了遗传多样性分析。选用10个多态性高、分辨力强的ISSR引物分别对供试材料基因组DNA进行扩增,共获得121个位点,其中多态位点带98个,多态位点比率(PPB)为81.0%。品种(系)之间的遗传相似系数变化范围在0.590 9~0.967 4之间。UPGMA聚类分析结果表明,在GS值为0.792的水平上供试材料可划分为7大类,其亲缘关系远近与地理来源关系不大且与香味类型没有必然的联系。
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| 关 键 词： | ISSR分子标记 凤凰单丛茶 遗传多样性 |
Genetic Diversity of Fenghuang-Dancong Tea Plant Germplasms by ISSR Markers |
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| WU Qinghan,ZHUANG Donghong,ZHU Hui,ZHAO Qingfang,YANG Peikui and MA Ruijun.Genetic Diversity of Fenghuang-Dancong Tea Plant Germplasms by ISSR Markers[J].Chinese Journal of Tropical Crops,2015,36(3):499-503. |
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| Authors: | WU Qinghan ZHUANG Donghong ZHU Hui ZHAO Qingfang YANG Peikui and MA Ruijun |
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| Institution: | Department of Biology, Hanshan Normal University;Department of Biology, Hanshan Normal University;College of Life Sciences, Northwest Normal University;Department of Biology, Hanshan Normal University;Department of Biology, Hanshan Normal University;Department of Biology, Hanshan Normal University |
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| Abstract: | ISSR molecular markers were used to analyze the genetic polymorphism of 48 Fenghuang-Dancong tea plant germplasms. Using 10 primers, we amplified 121 bands, of which 98 bands are polymorphic, and the average percentage of polymorphic bands was 81.0%. The genetic similarity among all the tested tea plant germplasms ranged from 0.590 9 to 0.967 4. Based on the genetic similarity and the UPGMA cluster, all the 48 accessions were clustered to 7 groups on the genetic similarity level of 0.792. The dengrogram indicated that the genetic of 48 tested tea plant germplasms had no certain relation with the geographical origin and aroma type. |
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| Keywords: | ISSR markers Fenghuang-Dancong Tea Genetic diversity |
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