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V. Charlesworth 《Equine Veterinary Education》2018,30(4):192-196
This report describes the clinical, biochemical and histopathological findings of a case of disseminated, primary mycobacterial infection with Mycobacterium bovis in a pony presenting with neurological signs and weight loss. This report revisits tuberculosis as a potential differential diagnosis in horses at a time of relatively strong tuberculosis control in the UK and iterates the importance of post‐mortem examination as a diagnostic tool for clinicians. Key public health questions following exposure to mycobacterial pathogens are also discussed. 相似文献
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Oral infection of goats with Mycobacterium avium subsp. hominissuis (MAH) resulted in a large variety of granulomas in organized gut-associated lymphatic tissues and intestinal lymph nodes. To characterize the cellular composition of granulomas, CD4+, CD8+, γδ, B lymphocytes and plasma, CD25+, CD68+, MHC-II+, Ki67+ and endothelial cells were labeled in consecutive frozen sections by immunohistochemistry and acid fast bacilli (AFB) by Kinyoun stain. Granulomas with extensive necrosis, little mineralization and variable numbers of AFB surrounded by many CD4+ T cells, but only few epitheloid macrophages were observed in severely sick goats at 2–3 mpi. They were interpreted as exuberant immune reaction. Organized granulomas with very few AFB were seen in clinically healthy goats at 13 mpi. The necrotic cores were surrounded by a zone of granulomatous infiltrate with many epitheloid macrophages and few lymphocytes. This zone was initially wide and highly vascularized and became progressively smaller. It was enclosed by an increasing layer of connective tissue. All organized granulomas were surrounded by compartimentalized tertiary lymphoid tissue. The granulomas in experimental infection of goats with MAH reflect the heterogeneity of lesions seen in mycobacterial infections of humans and ruminants and are therefore valuable for comparative research. 相似文献
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Novotny L Halouzka R Matlova L Vavra O Bartosova L Slany M Pavlik I 《Journal of fish diseases》2010,33(12):947-955
Mycobacteriosis in fish is a chronic progressive ubiquitous disease caused by Mycobacterium marinum, M. gordonae and M. fortuitum in most cases. The aim of this study was to describe the morphology and distribution of lesions in 322 freshwater ornamental fish across 36 species. Granulomatous inflammation was diagnosed by gross examination and histopathology testing in 188 fish (58.4%); acid‐fast rods (AFR) were determined in only 96 (51.1%) fish from 19 species after Ziehl–Neelsen staining. The most often affected organs with AFR were the kidney (81.2%), digestive tract (54.1%), liver (48.2%), spleen (45.9%) and skin (21.2%); sporadically, AFR were found in the branchiae (9.4%) and gonads (4.7%). In 14 randomly selected fish originating from four different fish tanks, the distribution of mycobacterial infection was studied by culture examination of the skin, gills, muscle tissue, digestive tract, liver, spleen and kidney. In 12 fish, the species M. marinum, M. gordonae, M. fortuitum, M. triviale, and M. avium subsp. hominissuis (serotypes 6 and 8 and genotype IS901? and IS1245+) were detected; mixed infection caused by different mycobacterial species was documented in five of them. 相似文献
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Allysa L. Cole Natalie M. Kirk Leyi Wang Chien-Che Hung Jonathan P. Samuelson 《Journal of veterinary diagnostic investigation》2022,34(1):116
Two aborted Chester White pig fetuses were presented to a veterinary diagnostic laboratory in Illinois. Postmortem examination identified no gross abnormalities. Histologic evaluation revealed multifocal necrosis of chorionic epithelial cells, coalescing areas of mineralization in the placenta, and focal accumulations of viable and degenerate neutrophils in the lung. Intra- and extracellular acid-fast bacilli were identified in the lesions in both the placenta and lungs. Bacterial culture of stomach contents yielded heavy growth of Mycobacterium fortuitum, a rapidly growing nontuberculous mycobacterium (NTM), which was further confirmed through whole-genome sequencing. NTM are opportunistic pathogens commonly found in the soil and in contaminated water supplies. In animals, M. fortuitum is typically introduced through cutaneous wounds leading to infections limited to the skin, with systemic infection being uncommon. To our knowledge, abortion caused by M. fortuitum has not been reported previously. 相似文献
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Keeping fish is a popular pastime in South Africa and the majority of ornamental fish are imported. A pilot study was initiated
to examine the health status of ornamental freshwater fishes imported to South Africa. Four groups of thirty fish each were
examined for the presence of external parasites, and processed for virus isolation and for bacterial and mycobacterial culture.
The groups consisted of goldfish (Carassius auratus), koi (Cyprinus carpio), guppies (Poecilia reticulata) and cardinal tetras (Cheirodon axelrodi). Mycobacterium fortuitum was isolated from the goldfish and koi. No other significant bacteria were isolated and virus culture was negative for all
groups. Skin scrapings and wet gill preparations were made to detect external parasites. Parasites were identified from fixed
material. External parasites included Trichodina mutabilis, Ichthyophthirius multifiliis, ciliophorans of the genus Tetrahymena, and monogeneans belonging to the genera Dactylogyrus and Gyrodactylus. This is the first report of Trichodina mutabilis in South Africa. Diseases imported with ornamental fish pose a risk to both indigenous fish populations and the aquaculture
industry.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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Real‐time PCR and immunohistochemistry (IHC) assays were developed to detect fish mycobacterial infections at the genus level, based on the RNA polymerase β subunit (rpoB) gene and polyclonal anti‐Mycobacterium rabbit serum, respectively. The PCR assay positively identified a number of pathogenic mycobacteria including Mycobacterium abscessus, M. avium ssp. avium, M. bohemicum, M. chelonae ssp. chelonae, M. farcinogenes, M. flavescens, M. fortuitum ssp. fortuitum, M. gastri, M. gordonae, M. immunogenicum, M. malmoense, M. marinum, M. montefiorense, M. phlei, M. phocaicum, M. pseudoshottsii, M. salmoniphilum, M. senegalense, M. shottsii, M. smegmatis, M. szulgi and M. wolinskyi. A detection limit equivalent to 102 cfu g?1 was registered for M. salmoniphilum‐infected fish tissue. The IHC precisely localized both free and intracellular mycobacteria in tissues and detected mycobacterial infections down to 102 cfu g?1 tissue. Both assays were found to be more sensitive than Ziehl–Neelsen (ZN) staining, where the detection limit was below 8 × 103 cfu g?1 tissue. Although specificity testing of the real‐time PCR against a panel of non‐Mycobacterium spp. revealed a degree of cross‐reaction against pure DNA extracted from Nocardia seriolae and Rhodococcus erythropolis, no cross‐reactions were identified (by either real‐time PCR or IHC) on testing of formalin‐fixed paraffin‐embedded (FFPE) tissues confirmed to be infected with these bacteria. The broad applicability of both assays was confirmed by analysis of FFPE tissues from a range of fish species infected with diverse Mycobacterium spp. The results indicate that both assays, alone or in combination, constitute sensitive tools for initial, rapid diagnosis of mycobacteriosis in fish. This should in turn allow rapid application of more specific studies, i.e. culture based, to identify the specific Mycobacterium sp. involved. 相似文献
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本试验对从吉林省不同区域的7个城市采集的503份牛颌下淋巴结样品和497份牛肠系膜淋巴结样品进行非结核分枝杆菌的流行情况的调查,样品均采自结核(副结核)变态反应阴性牛只,调查中得到阳性样品25份,阳性率2.5%,其中颌下淋巴结阳性率1.59%,肠系膜淋巴结阳性率3.42%,成功分离扩增培养出14株非结核分枝杆菌,并进行了分类鉴别,得到母牛分枝杆菌1株,龟分枝杆菌脓肿亚种2株,苏加分枝杆菌2株,偶发分枝杆菌1株,蟾蜍分枝杆菌6株,胞内分枝杆菌2株.调查结果表明:非结核分枝杆菌单独感染的情况不容乐观,应加强对非结核分枝杆菌的检验检疫工作. 相似文献