A Potential Screening System for Identifying Sorghum Ecotypes with Increased Lysine in the Seeds

Classical “high-lysine” sorghum lines are characterized by smaller seeds than average, due to a decrease in prolamin synthesis and a subsequent decrease in yield. To exploit the natural variation in lysine content and to identify ecotypes with a seed lysine content higher than average, characterized by plump seeds, a method was developed based on root-growth inhibition of seeds growing on a medium containing aminoethylcysteine (AEC), a lysine analogue. By using a collection of sorghum mutants and ecotypes a correlation coefficient of 0.926 between root length and lysine content was established. This method, which uses the root length of plants growing on aec to indicate which lines have a potential elevated lysine content, can be applied for the screening of sorghum germplasm. Since this is a non-destructive method it can also be used at the individual seed level, for example for screening progenies of regenerated plants from in vitro culture to exploit the somaclonal variation.     

Growth,Meat Yield,and Economic Responses of Broilers Provided Three- and Four-Phase Schedules Formulated to Moderate and High Nutrient Density During a Fifty-Six-Day Production Period

This study examined growth responses and meat yield of broilers provided a 3- or 4-phase feeding schedule formulated to moderate or high nutrient density. Four dietary treatments were implemented consisting of high or moderate nutrient density diets that were provided for the duration of 3 phases (1 to 17, 18 to 35, and 36 to 56 d) or 4 phases (1 to 17, 18 to 35, 36 to 46, and 47 to 56 d).Provision of a 3-phase schedule did not alter growth performance, meat yield, or diet cost per final BW. Broilers receiving high nutrient diets had improved growth rate and feed conversion from 1 to 35 d. In the 3-phase schedule, birds given the high nutrient density diets had advantages in BW gain and pectoralis minor breast weight. Market changes in meat prices impact differences in gross feeding margins between diets formulated to high or moderate nutrient density more than variation in diet cost.     

赖氨酸占蛋白质及饲粮的比例对杜洛克猪生长肥育性能及胴体品质的影响

杜洛克猪48头,体重27kg左右,分6组。试验前期(27～60kg)日粮的消化能均为13.67MJ)/kg,后期(60～90kg)均为13.44MJ/kg。粗蛋白1、2、3组前期均为18％,后期均为16％;4、5、6组前期均为16％,后期均为14％。同时配合3种赖蛋比水平6.6～6.9％,6.1～6.25％及5％。试验结果表明:4、5、6组平均日增重、饲料利用率及胴体瘦肉率都略高于1、2、3组,但差异不显著(P>0.05)。在前期,以第5组平均日增重最高(达782克),极显著高于其他各组(P<0.01);饲料利用率2.41,极显著好于其他各组(P<0.01)。后期以第5组平均日增重最高(875克)。试验前期,第5组获得最佳的日增重(822克),极显著高于其他各组(P<0.01),饲料利用率2.98,胴体瘦肉率65.79％,与其他各组差异不显著(P>0.05)。在两种粗蛋白水平组内,均以赖蛋比6.1～6.25％的第5组和第2组生长速度最快(822克和778克)。说明在相同的能量水平下,赖蛋比是影响生长速度的主要因素。     

日粮纤维及其对幼龄单胃动物消化功能的影响

日粮纤维能提供大量的能量,而且有利于维持动物机体的正常消化功能。本文综述了日粮纤维的代谢及对幼龄单胃动物消化功能的影响。     

赖氨酸采食量对早期断奶仔猪生长发育及胰腺内分泌机能的影响

将55头21日龄断奶的雄性仔猪随机分为5组，各组仔猪饲喂赖氨酸含量为0．60％、0．80％、1．00％、1．20％和1．40％的试验日粮21d。42日龄结束饲养试验时进行葡萄糖灌注试验，观察赖氨酸采食量对早期断奶仔猪生长发育和胰腺内分泌的影响。结果表明：早期断奶仔猪体增重与赖氨酸采食量为强正相关，根椐体增重指标，21～42日龄阶段，仔猪的赖氨酸采食量不应低于3．16g／d，日粮赖氨酸含量不应低于1.40％。赖氨酸严重缺乏(采食量小于1．45g／d)时，胰岛素的分泌量下降，对高血糖应激的反应能力降低。     

Effect of feed intake level and dietary protein content on the body temperature of pigs housed under thermo neutral conditions

Feed intake and diet composition appear to affect the body temperature of pigs. Two trials were conducted to analyse the effect of feed intake level and dietary protein content on the intestinal temperature (IT) of pigs housed under thermo neutral conditions. Ten pigs (64.1 ± 1.3 kg initial body weight) fitted with an ileal cannula were used. A thermometer set to register the IT at 5‐min intervals was implanted into the ileum through the cannula. In both trials, the ambient temperature ranged from 19.1 to 21.6°C and the pigs were fed at 07:00 and 19:00 hr (same amount each time). In trial 1, the pigs were fed daily 1.2 or 1.8 kg of a wheat–soybean meal diet. The IT followed a similar pattern along a 24‐hr period regardless the feed intake level. The IT rapidly increased up to 0.61 and 0.74°C after the morning meal and up to 0.53 and 0.47°C after the evening meal in pigs fed 1.2 and 1.8 kg/d respectively. The postprandial IT was higher in pigs fed 1.8 kg after each meal (p < .05). In trial 2, pigs were fed daily 1.8 kg of a low (11%) or a high (22%) crude protein diet. The IT followed a similar pattern along the 24‐hr period regardless the dietary protein level. The postprandial IT did not differ between pigs fed the low protein or the high protein (p > .10). The IT rapidly increased up to 0.66 and 0.62°C after the morning meal in pigs fed the high‐ and low‐protein diet (p < .05), but there was no change after the evening meal (p > .10). In conclusion, the feed intake level affected the IT of pigs housed under TN conditions, but the dietary protein content had no effect.     

南方某矿区土壤镉污染及作物健康风险研究

为了评估南方某县土壤镉污染情况、量化当地不同作物镉含量并对不同人群健康风险进行评估，从该县3个镇采集135份土壤样本，入户采集128份自产大米样本，种植区采集7份脐橙样本，对其中镉含量进行测定。结果表明，3镇采集土壤样品镉超标率分别为41.67%、28.81%和21.62%，地累积指数计算得到该县68.9%的土壤样本受到不同程度的镉污染，大米镉含量超标率分别为48.57%、34.48%、8.82%。而脐橙果肉样品中镉含量较低，远低于0.05 mg·kg^-1。计算不同作物的危害商数（HQ）可得，食用自产大米的危害商数（HQ）值普遍大于1，风险较高，而食用脐橙果肉产生的健康风险低。种植脐橙具有一定的经济效益，且当地具有脐橙适宜的生长条件，故考虑在重污染区种植脐橙取代种植水稻。     

Effect of Lysine Supplement on the Digestion and Metabolism of the Rumen in Small-tail Han Sheep

5 male Small-tail Han sheep with the body weight of 45 kg at 1.5 years old,fitted with permanent rumen fistula,to study of the effect of the supplement of the untreated commercial lysine on the rumen microbiocoenosis,rumen metabolism and whole body metabolism by 5×5 Latin square design (5 groups:control,control with low urea (equal nitrogen),low lysine,control with high urea (equal nitrogen),high lysine).The amounts of lysine-HCl supplement were 0,4.0 and 8.0 g/kg diet (as DM basis) in each group respectively,with two urea supplement control groups of equal amount of nitrogen.The results showed that by supplement of 4.0 and 8.0 g/kg of lysine-HCl diet (as DM basis) respectively,the voluntary intake of dry matter of sheep were increased by 5.5%(P>0.05) and 11.8%(P<0.05) respectively.The total amount of rumen bacteria were increased by 18.9%(P<0.01) and 23.9%(P<0.01) respectively,as the amount of rumen Coccus was increased by 21.2%(P<0.01) and 30.1%(P<0.01) respectively.The amount of rumen big Bacillus was decreased by 16.7%(P<0.05) and 33.3%(P<0.01) respectively.The total VFA of was increased by 9.6%(P<0.05) and 12.2%(P<0.01) respectively,as the butyric acid was increased by 12.8%(P>0.05) and 20.2%(P<0.01) respectively.The apparent digestibility of organic matter was increased by 6.4%(P>0.05) and 10.0%(P<0.05) respectively,the apparent digestibility of crude protein was increased by 7.0%(P>0.05) and 18.3%(P<0.01) respectively;And nitrogen retention was increased by 46.4%(P>0.05) and 110.7%(P<0.01) respectively.However,there was no effect of lysine supplement on the amounts of the rumen protozoa and fungi,and the concentration of rumen ammonia nitrogen.It was concluded that the supplement of untreated commercial lysine to adult sheep would increase the voluntary intake,digestibility,nitrogen retention the rumen microbiocoenosis and the total amount of rumen bacteria,especially the rumen Coccus of adult sheep.The effect of lysine supplement could not be replaced by urea supplement completely.