1株山羊源海藻糖比伯斯坦杆菌的分离鉴定及药敏试验

为确定引起山羊肺炎的病原,本研究从病死山羊肺脏中分离纯化获得1株细菌,通过生化试验和分子生物学方法鉴定为海藻糖比伯斯坦杆菌。利用生物信息学软件MEGA6.0对巴氏杆菌科5个属细菌的白细胞毒素基因(lktA)和RNA聚合酶β亚基基因(rpoB)分别构建遗传系统发育树,可知分离株与海藻糖比伯斯坦杆菌(AF314526.2、CP003745.1、CP006955.1等)的lktA基因相似性最高,位于同一簇内;分离株和海藻糖比伯斯坦杆菌(CP006956.1、JACI01000002.1、CP003745.1等)的rpoB基因聚类为一簇,相似性最高;而与和葡萄糖苷曼氏杆菌(KU986490.1)的rpoB基因相似性最低。药敏试验显示,该菌对头孢噻肟、头孢他啶和环丙沙星等抗菌药物最敏感,对多西环素、阿莫西林和林可霉素等耐药。本研究为该菌致病机理的研究与疫苗的研发奠定了基础,也为临床用药提供了参考依据。     

Role of Bibersteinia trehalosi, respiratory syncytial virus,and parainfluenza-3 virus in bighorn sheep pneumonia

Pneumonic bighorn sheep (BHS) have been found to be culture- and/or sero-positive for Bibersteinia trehalosi, respiratory syncytial virus (RSV), and parainfluenza-3 virus (PI-3). The objective of this study was to determine whether these pathogens can cause fatal pneumonia in BHS. In the first study, two groups of four BHS each were intra-tracheally administered with leukotoxin-positive (Group I) or leukotoxin-negative (Group II) B. trehalosi. All four animals in Group I developed severe pneumonia, and two of them died within 3 days. The other two animals showed severe pneumonic lesions on euthanasia and necropsy. Animals in Group II neither died nor showed gross pneumonic lesions on necropsy, suggesting that leukotoxin-positive, but not leukotoxin-negative, B. trehalosi can cause fatal pneumonia in BHS.     

山羊海藻百伯史坦菌分离鉴定与药敏试验

为了对陕西省某羊场羊群发生呼吸道疾病进行确诊,取病死羊肺脏进行细菌培养、分离、纯化、生化试验、16S rRNA测序、动物致病性试验以及药敏试验.结果表明,分离株的培养特性与生化试验结果与山羊海藻百伯史坦菌符合;16S rRNA比对结果显示分离细菌与山羊海藻百伯史坦菌同源性为98％;致病性试验表明山羊海藻百伯史坦菌对小鼠...