Identification by isoelectric focusing of creatine kinase-MM isoforms in the plasma and striated muscle of pigs

Total creatine kinase (CK) and CK-MM isoforms were determined in plasma and longissimus dorsi muscle extracts from normal pigs. Based on their total CK activity, the pigs were divided into two groups. Pigs of group 1 (n=16) had a mean plasma total CK of 298±16 U/L and the distribution of the CK-MM isoforms was 65.7±2.5% CK-MM₃, 18.9±1.6% CK-MM₂ and 15.3±1.5% CK-MM. In group 2 (n=18; 826±75 U/L total CK) four isoforms were observed: 3.1±0.9% CK-MM, 67.9±3.0% CK-MM₃, 21.5±2.3% CK-MM₂ and 7.5±1.3% CK-MM₁. The differences between the two groups of pigs were significant (p<0.001) for CK-MM₁ and the presence of CK-MM. Four CK-MM isoforms were also detected in longissimus dorsi muscle homogenates: 45.6±8.1% CK-MM, 32.6±11.7% CK-MM₃, 16.6±2.3% CK-MM₂ and 5.1±2.8% CK-MM₁. The release of CK-MM isoforms from muscle into plasma seems to be unrelated to the concentration of these isoforms in striated muscle.     

大白菜一代杂种及双亲幼苗的等电聚焦电泳过氧化物同工酶分析

等电聚焦电泳是近些年发展起来的分离蛋白质的有效生化技术．操作简便，分辨率高，电泳条带多而且细，信息量大，利于进行深入细致的研究。该文利用等电聚焦丙烯酰胺电泳方法分析了6个大白菜一代杂种及双亲幼苗的过氧化物同工酶电泳图谱，共分离出十几条酶带，F1杂种与亲本间同工酶图谱均表现出明现差异，特别是A鉴别区内的酶带差异最明显，大多为互补型，是主要鉴别区。进一步研究结果表明，6个大白菜品种均可利用过氧化物同工酶鉴别由自交引起的假杂种。利用这项技术大量开展了这6个品种的商品种子纯度检测工作，同时在田间进行了验证性测定，二者结果基本相符。     

NUMERICAL SOLUTIONS OF SHOCK WAVE FOCUSING WITH CCW METHOD

By the use of a numerical scheme of chisnell-Chester-Whitham Method (CCW), some numerical solutions to the problems of shock wave focusing are completed successfully and simply.     

牧草干燥太阳能聚焦吸收器机理分析与优化

目前太阳能干燥设备是以非聚焦板式空气集热器为关键工作部件,其干燥效率和生产率均较低。为此,研究了太阳能聚焦吸收器的黑腔功能,利用该功能减少聚焦光线对外二次辐射和能量损失。设计4种结构类型的太阳能聚焦吸收器,进行了性能机理研究和对比分析,其中三角形聚焦吸收器呈现出良好的黑腔效果。进一步对三角形聚焦吸收装置进行优化,得出吸收器内腔深度在4 cm左右时性能最佳。对4种吸收器进行了试验测试,结果表明,三角形优于圆弧形,圆弧形优于方形,性能最差的是半圆形。吸收器开口宽度为5 cm时的黑腔效果低于开口宽度为3 cm时的效果。盖板形状对吸收器性能产生影响,平板优于圆形,V形最差。     

Isolation of pituitary glycoprotein gonadotropins from the grass carp (Ctenopharyngodon idell)

Gonadotropins (GTH) were isolated from pituitary glands of grass carp and their properties investigated. Acetone-dried pituitaries were extracted with ammonium acetate-ethanol and were then passed through cation exchanger, anion exchanger, and gel filtration column. Two glycoprotein gonadotropins (DE I and DE II) were isolated of MW 56,000 and 53,000 daltons, respectively, as determined by gel filtration technique. Isoelectric points of the two GTHs also differed from each other. Sialic acid contents were 3.64% and 1.47% for DE I and DE II, respectively. Both GTH's stimulated, in a similar manner, testicular androgen and ovarian estradiol synthesisin vitro.     

水牛梭形住肉孢子虫抗原的薄层等电聚焦行为分析

应用薄层聚丙烯酰胺等电聚焦电泳（ＩＦＥ）对水牛梭形住肉孢子虫（Ｓａｒｃｏｃｙｓｔｉｓｆｕｓｉｆｏｒｍｉｓ）抗原进行了分析。结果表明，经ＳｅｐｈａｄｅｘＧ－１００纯化的包囊抗原在电泳图谱上显示３条相距较近的带，等电点分别为６．３０，６．４５，６．６５；缓殖子纯化抗原仅出现１条带，等电点为６．４０     

桃水溶性蛋白质等电聚焦电泳分析初报

对不同 pH 范围的两性载体电解质(Ampholine)进行等电聚焦(IEF)电泳,能获得不同的水溶性蛋白质谱型.结果表明:1.桃大多数器官酸性蛋白质含量丰富,宜选用pH3.5—7.0的 Ampholine 进行 IEF 电泳.2.桃不同器官水溶性蛋白质谱型差异显著.宜用花药、幼叶、叶柄、花瓣作为 IEF 电泳的主要分析器官;成熟叶、萼筒、种子作为辅助分析器官.3.萼筒和花瓣水溶性蛋白质谱型可鉴别黄肉桃、白肉桃两类品种,而花瓣谱型亦能区分桃大花、小花两种类型.     

Identification of tomato cultivars (Lycopersicon esculentum) by polyacrylamide isoelectric focusing

Summary Isozyme analyses have been used for the definitive identification of many plant cultivars, but not for cultivated tomatoes. Six isozyme systems, namely alcohol dehydrogenase, acid phosphatase, phosphoglucomutase, esterase, phosphoglucoisomerase and 6-phosphogluconate dehydrogenase of tomato seed extracts were resolved by isoelectric focusing on polyacrylamide gels with a narrow pH gradient. Nine alcohol dehydrogenase phenotypes were distinguished which, with three acid phosphatase phenotypes, identified twelve of the seventeen cultivars. Fewer differences were found for the other isozymes. Since this method could differentiate between breeding parents and their progeny it is concluded that further investigations are warranted.Abbreviations APS acid phosphatase - ADH alcohol dehydrogenase - EST esterase - IEF isoelectric focusing - PGI phosphoglucoisomerase - PGM phosphoglucomutase - 6-PGDH 6-phosphogluconate dehydrogenase - RFLP restriction fragment length polymorphism - VOPRI Vegetable and Ornamental Plant Research Institute     

Inheritance of peroxidase isozymes in mulberry (Morus spp.)

Summary Electrophoretic variants of peroxidase in mulberry (Morus spp.) were demonstrated by thin-layer gel isoelectric focusing. Of these variants, three isozyme band groups were found to be controlled by codominant alleles at a single locus. The gene symbol Px ₁ was given to this locus, with alleles Px ₁ ¹ and Px ₁ ² assigned to the A6-A7-A8 and A7-A8-A9 band groups, respectively. The A6-A7-A8-A9 band group proved to be controlled by the Px ₁ ¹ and Px ₁ ² heterozygote.Additional experiments showed that among the three banding types, there were no statistically significant differences in leaf blade length, leaf blade width, length-width ratio of leaf blade, internode length, phyllotaxis, leaf shape, tree vigor and resistance to powdery mildew, but there were significant differences in leafstalk length.     

The electrofocusing composition of the Large and Small Sub-unit polypeptides of Fraction-1-Protein in rice x wheat and rice x sorghum hybrids

Summary The polypeptide composition of Fraction-1-Protein (F1P) from rice × sorghum, rice × wheat hybrids and their respective parents have been analyzed by a microelectrofocusing method. The large sub-unit (LSU) is composed of three polypeptides and the small sub-unit (SSU) of two polypeptides in rice and sorghum parents and rice × sorghum hybrids. Similarly, LSU is composed of three polypeptides in the rice and wheat parents and rice × wheat hybrids. Two polypeptides occur in the SSU of rice parent and rice × wheat hybrids where as only one polypeptide in the wheat parent. These polypeptides also differ in their isoelectric points. Based on the previous reports of F1P inheritance in hybrids in other crops, F1P analysis of rice × sorghum and rice × wheat hybrids does not seem to be an important marker to identify such intergeneric hybrids. Since this is first such report of F1P inheritance in hybrids between distantly related plants, its implication in different modes of inheritance are discussed.Abbreviations F1P Fraction-1-Protein - IEF Isoelectric focusing - pI Isoelectric points - LSU Large sub-unit - RuBPCase Ribulose 1,5-Bisphosphate Carboxylase-oxygenase - SSU Small sub-unit