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Neurospora crassa对三唑醇的抗药性分子机制研究 总被引:5,自引:0,他引:5
通过紫外光诱变获得Neurospora crassa对三唑醇杀菌剂抗性突变体"Nc70"。用2.5μg/ml三唑醇处理,突变体内甲基甾醇与脱甲基甾醇比例变化幅度远远小于亲本敏感菌株"STA"。突变体对三唑醇的吸收能力与敏感菌株相似,但药剂在体细胞内积累较少。三唑醇处理N.crassa 2 h后,药剂在菌体内的含量趋向稳定,突变体内的药量较敏感菌体低56.1%,而麦角甾醇合成量则高于敏感菌株53.7%。 相似文献
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[目的]进一步试验IAA和NAA分别与顶向传导的杀菌剂三唑醇进行耦合的耦合物在大豆苗中的传导性。[方法]用耦合物药液对大豆苗进行喷雾处理,色谱法检测耦合物在大豆苗各部位的含量。[结果]NAA-三唑醇和三唑醇均不能向下传导至根部,而IAA-三唑醇却具有双向传导的特性。0.5 mmol/L IAA-三唑醇处理大豆苗叶片后12 h检测到根部含量为1.87μg/g(FW),高于此时茎中的含量0.68μg/g,但与同浓度的IAA混合施用时,根中IAA-三唑醇的含量减少到0.80μg/g。[结论]耦合物中IAA基团能引导耦合物向下传导。 相似文献
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3种杀菌剂对日本青鳉的急性毒性研究 总被引:3,自引:0,他引:3
[目的]探讨多菌灵及三唑类杀菌剂对水生生物的毒性.[方法]以不同鱼龄的日本青鳉(Oryzias latipes)为研究对象,采用半静态暴露法,测定了多菌灵、三唑醇、三唑酮3种杀菌剂对日本青鳉的急性毒性.[结果]多茵灵、三唑醇、三唑酮3种杀菌剂对日本青鳉成鱼、幼鱼、仔鱼的LC50(96 h)值分别为:0.749 8、0.4646、0.0512 mg/L;20.5415、14.4866、1.4735 mg/L;12.4707、11.0202、0.3536mg/L.单一杀菌剂对日本青鳉不同鱼龄毒性高低依次为:仔鱼>幼鱼>成鱼;3种杀菌剂之间毒性高低依次为:多菌灵>三唑酮>三唑醇.[结论]为相关部门制定3种杀菌剂的使用标准提供了理论依据. 相似文献
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生长素介导下三唑醇在大豆植株内的传导性(英文) 总被引:4,自引:0,他引:4
[Objective] This study was to investigate the mobility of IAA-triadimenol and NAA-triadimenol in soybean seedlings. [Method] Soybean seedlings were treated by spraying the solution of conjugated compounds onto their leaves; and chromatography was employed to measure the contents of IAA-triadimenol and NAA-triadimenol in different parts of these soybean seedlings, through which to represent the mobility of the conjugates. [Result] Both the triadimenol and NAA-triadimenol could not transport basipetally; whereas IAA-triademenol was ambimobile. When sprayed with 0.5 mmol/L IAA-triadimenol, as much as 1.87 μg/(g·FW) IAA-triadimenol was detected in the roots of soybean seedlings 12 h later, which was higher than that in the stem [0.68 μg/(g·FW)]; while that decreased to 0.80 μg/(g·FW) in the seedlings sprayed with 0.5 mmol/L IAA-triadimenol and the same concentration of IAA. [Conclusion] IAA moiety of the conjugate could enhance the transport and accumulation of the fungicide towards the root via the IAA carriers. 相似文献
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建立一种利用超高压液相色谱串联质谱同时测定猪肉、鸡肉、鸡蛋和牛奶中三唑酮和三唑醇残留的分析方法,其中使用乙腈提取,GC/NH_2固相萃取小柱净化。结果显示:建立的分析方法线性、精确度和准确度满足SANCO/12571/2013要求,灵敏度满足CAC要求,而且操作步骤简单,耗时短,对大批量样品来说大大提升了检测效率。 相似文献
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