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This work reports the transferability and polymorphism of previously reported SSRs in 10 Prunus species. The availability of a large number of SSRs in the genus Prunus makes marker choice random, while preventing comparison of results in fingerprinting studies. The availability of SSR markers, polymorphic in a wide sample of Prunus species, would facilitate marker choice, while allowing the comparison of results. In this work, microsatellite markers useful for analyzing 10 different Prunus species (P. persica, P. dulcis, P. armeniaca, P. domestica, P. insititia, P. salicina, P. cerasifera, P. avium, P. cersus and P. mahaleb) were searched through screening SSRs previously reported to be conserved and/or polymorphic in more than one Prunus species. A selected group of 13 SSRs, transferable to the 10 species, was analyzed in terms of their usefulness for analyzing these species. The amplification range, polymorphism and variability detected by these loci are reported. The information provided will be useful for Prunus genetic studies as well as conservation and management of Prunus germplasm resources. 相似文献
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本研究对50条甘蓝SSR引物在其近缘种青花菜中的通用性进行了分析,结果表明,38对引物在青花菜上可有效扩增,扩增产物分子量在100~1500bp,有效扩增比率为76%,其中18%具有较好的多态性,揭示了两种作物基因组间存在一定的相似性。同时利用获得的多态性较好的9对引物对青花菜进行基因型鉴定和遗传多样性分析,20份青花菜基因型中共检测到51个基因位点,平均每个引物组合可扩增出5.67个条带,多态性为66.7%。多引物组合可鉴别出所有青花菜基因型。聚类分析结果表明同一来源的基因型间具有相近的遗传基础,且聚类与熟性具有一定的相关性。本研究为今后青花菜种质资源的收集、利用及分子标记辅助育种提供了新的SSR标记和参考。 相似文献
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Xiao-Ping Jia Yun-Su Shi Yan-Cun Song Guo-Ying Wang Tian-Yu Wang Yu Li 《Genetic Resources and Crop Evolution》2007,54(2):233-236
The development of EST-SSR in foxtail millet (Setaria italica) for polymorphism and transferability study was reported here. From 1213 EST sequences, 30 SSRs were obtained and primers
were designed for 26 SSRs. Among them, four pairs of SSR primers amplified polymorphic products in 12 foxtail millet cultivars
and one accession of Setaria viridis, a wild relative of foxtail millet, with 10 alleles detected for the four loci and 2.5 alleles per locus. In addition, ten
SSR markers could be transferred to other nine Gramineae species. The putative functions of 11 ESTs containing polymorphic
and transferable SSRs were also identified. 相似文献
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M. L. Wang Z. B. Chen N. A. Barkley M. L. Newman W. Kim P. Raymer G. A. Pederson 《Genetic Resources and Crop Evolution》2006,53(4):779-791
One hundred and thirty SSR markers from wheat, maize and sorghum were screened for the transferability to Paspalum. The transfer rate was 67.5, 49.0 and 66.8% respectively. This would be a very efficient approach for DNA marker development
for species which are not well studied molecularly. The polymorphism level for transferred SSR markers was 51.5% within species
(Paspalum vaginatum) and 87.1% among Paspalum species. The high level of polymorphism is directly related to the high degree of heterozygosity maintained by its way of
reproduction, i.e. self-incompatibility. Forty transferred polymorphic SSR markers were selected and used for characterization
and evaluation of seventy-three Paspalum accessions. In total, 209 polymorphic bands were detected from these 40 SSR markers, with an average of five polymorphic
bands per marker. The Paspalum accessions clustered into three major groups. Two very similar dendrograms can be generated from either 109 or 209 polymorphic
bands. This led us to determine that 18 of the transferred SSR markers were sufficient for genetically differentiating the
investigated germplasm accessions. The number of SSR markers required for germplasm characterization and evaluation is discussed.
This is the first report of the transfer of SSR markers from major field crops to newly emerged environmental turfgrasses. 相似文献
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为研究虾夷扇贝(Patinopecten yessoensis)和栉孔扇贝(Chlamys farreri)的群体遗传多样性并分析其亲缘关系,对在虾夷扇贝中开发的60个EST-SSR位点在栉孔扇贝中的通用性进行了研究。结果显示,21个位点可在栉孔扇贝中扩增出特异性条带,通用性比例达35.00%,其中,17个位点具有多态性,多态性比例达28.33%。在虾夷扇贝和栉孔扇贝群体中,平均等位基因数(N_a)分别为2.7647和2.3529;平均有效等位基因数(N_e)分别为1.9487和1.6350;平均观测杂合度(H_o)为0.6314和0.3333;平均期望杂合度(H_e)为0.4569和0.3139;平均多态信息含量(PIC)为0.3726和0.2597;Nei's(1973)基因多样性指数(I)分别为0.4493和0.3087;Shannon信息指数分别为0.7176和0.5041;固定指数(F_(is))检测发现,7个位点偏离了Hardy-Weinberg平衡;遗传分化系数(F_(st))为0.2398。本研究开发的21对通用性EST-SSR标记,为进一步开展虾夷扇贝和栉孔扇贝遗传多样性分析、分子辅助育种、基因发掘和种质资源评价奠定了基础。 相似文献
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Young Hoon Park Seong Gyu Ahn Young Mi Choi Hye Jeong Oh Dong Chun Ahn Jin Gi Kim Jum Soon Kang Young Whan Choi Byoung Ryong Jeong 《Scientia Horticulturae》2010
The ‘Genome database for Rosaceae (GDR)’ provides a large collection of expressed sequence tags (ESTs) harboring simple sequence repeats (SSRs) from several Rosaceae genera, including Rosa (rose). Primer pairs flanking SSR were designed for 312 unique Rosa ESTs based on GDR database. Eight rose (Rosa hybrida L.) genotypes were tested for PCR amplification, and 287 (92%) of the primer pairs generated allele-specific PCR bands that were readily scored. From 183 (63.7%) primer pairs that evidenced polymorphic alleles among the eight rose cultivars, 20 pairs evidencing EST sequence homology to known gene functions and high levels of polymorphism were selected and utilized for DNA fingerprinting and genetic diversity assessments of 47 rose hybrids. A total of 202 polymorphic bands were scored and generated unique fingerprints for each rose hybrid. The Nei–Li genetic similarity coefficients among 1081 pair-wise comparisons of 47 cultivars exhibited a broad range of genetic variations from 0.30 (‘Grand King’ and ‘Carnival’) to 0.99 (‘First Red’ and ‘Red Champ’). UPGMA cluster analysis divided 47 hybrids into five major groups and two sub-groups. The cross-species transferability of 273 EST-SSR primer pairs was evaluated using four genotypes of the strawberry, a genus member of the Rosaceae family. PCRs on the DNA samples of strawberry were successful for 165 primer pairs; among these, 123 pairs amplified 243 polymorphic bands. As surrogates of the marker transfer, the phenetic relationship among the four strawberry genotypes was evaluated. Genetic similarity coefficients varied from 0.78 (‘Maehyang’ and ‘Janghyee’) to 0.64 (‘Janghyee’ and ‘Pragana’). The results of cluster analysis showed that the three octaploid strawberry cultivars were quite similar, whereas the diploid ‘Pragana’ was related distantly at the genomic DNA level. The EST-SSR markers developed in the present study can be efficiently utilized for genetic diversity studies in Rosaceae. 相似文献
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We report on the development of novel simple sequence repeat (SSR) markers from publicly available Cucumis sativus expressed sequence tags (ESTs) and on their transferability among related species. In total, 533 di- to penta-type SSRs were identified from 6344 cucumber ESTs retrieved from GenBank. Identified SSRs mainly comprised of di- and tri-nucleotide repeats, of which AG and AAG motifs were much abundant. A total of 392 SSR-containing unigenes (non-redundant ESTs/consensus sequences) were suitable for primer design. From these, 35 primer pairs were designed as representative samples and 28 were usable markers. Twenty-six out of 28 usable markers revealed polymorphism among 21 cucumber accessions with 2–7 alleles detected (mean = 3.77) and their polymorphism information content (PIC) values ranged from 0.091 to 0.748 (mean = 0.388). The polymorphism observed herein partially arose from the null alleles which occurred at the multiple homoeoloci detected by the markers. Transferability of the 28 EST-SSR markers was investigated in four other cucurbits: melon, watermelon, pumpkin and gourd which showed frequency of 92.9%, 57.1%, 53.6% and 60.7%, respectively. The EST-SSR markers developed herein will complement the currently available genomic SSR markers and may be useful for genetic studies in cucumber and related species. 相似文献
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Maristerra R. Lemes Thieme M. Martiniano Vanessa M. Reis Camila P. Faria Rogério Gribel 《Genetic Resources and Crop Evolution》2007,54(8):1653-1657
This study reports on the cross-species amplification of 23 microsatellite markers previously developed for Theobroma
cacao L. (Sterculiaceae), source of chocolate in three economically important Amazonian species of Theobroma (T. grandiflorum, T. subincanum, T. sylvestre). Thirteen of the 23 microsatellite loci tested were polymorphic across the three species at 2–13 alleles per locus. The
observed heterozygosity per locus varied from 0.18 to 0.84 and expected heterozygosity ranged from 0.28 to 0.87. The high
level of transferability and genetic information content of these microsatellite loci indicate their usefulness for population
genetic, mating system and breeding studies of these economically important Amazonian fruit trees. 相似文献