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| 西瓜和甜瓜的SSR引物对三种瓜类作物的通用性分析 | |
| 引用本文： | 唐鑫,张海英,许勇,程蛟文,杨衍,胡开林.西瓜和甜瓜的SSR引物对三种瓜类作物的通用性分析[J].分子植物育种,2011,9(6):760-764. |
| 作者姓名： | 唐鑫 张海英 许勇 程蛟文 杨衍 胡开林 |
| 作者单位： | 1. 华南农业大学园艺学院,广州,510640 2. 北京市农林科学院蔬菜研究中心,北京,100097 3. 中国热带农业科学院热带作物品种资源研究所,儋州,571737 |
| 基金项目： | 广东现代农业产业技术体系建设专项资金资助 |
| 摘 要： | 利用西瓜的1 824对SSR引物和甜瓜的259对SSR引物,对丝瓜、冬瓜和苦瓜的DNA进行了PCR扩增,分析了西瓜和甜瓜SSR引物对三种瓜类作物扩增的通用性以及三种瓜类作物中不同类型之间的多态性.结果表明,西瓜和甜瓜SSR引物对丝瓜、冬瓜和苦瓜的DNA扩增通用性分别是15.31％、23.04％和14.74％.但是,在三... |
| 关 键 词： | SSR 丝瓜 冬瓜 苦瓜 通用性 多态性 |
Comparison of Versatility and Polymorphism of Watermelon and Melon SSRs in Three Cucurbitaceae |
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| Tang Xin,Zhang Haiying,Xu Yong,Cheng Jiaowen,Yang Yan,Hu Kailin.Comparison of Versatility and Polymorphism of Watermelon and Melon SSRs in Three Cucurbitaceae[J].Molecular Plant Breeding,2011,9(6):760-764. | |
| Authors: | Tang Xin Zhang Haiying Xu Yong Cheng Jiaowen Yang Yan Hu Kailin |
| Institution: | 1 South China Agricultural University, GuangZhou, 510640; 2 Beijing Vegetable Research Center, BeiJing, 100097; 3 Tropical Resources Institute crop varieties, Chinese Academy of Tropical Agricultural Sciences, DanZhou, 571737 |
| Abstract: | In this study, by PCR amplification with the genomic DNA of towel gourd, wax gourd and bitter gourd, 1 824 watermelon SSR primers and 259 melon SSR primers were tested for analyzing their versatility and polymorphism of various types among three cucurbitaceae crops. The results indicated that the versatility of SSR primers amplification in towel gourd, wax gourd and bitter gourd was 15.31%, 23.04% and 14.74%, respectively. However, the polymorphism level of all these SSR primers among three cucurbitaceae crops and various types was very low. The ratio of polymorphism SSR primers between ridge gourd and sponge gourd, wax gourd and chieh-qua and among eight bitter gourds from different origins was 3.46%, 0.38% and 1.68%, respectively. |
| Keywords: | SSR Towel gourd Wax gourd Bitter gourd Transferability Polymorphism |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |