Current practices in many nurseries involve the germination of tropical rainforest seedlings in shaded conditions and transferral, at a later date, to environments with greater light intensities. Determination of the ability of these seedlings to acclimate to increased light intensities will allow seedling stock to be processed with maximum efficiency within the nursery. The acclimation abilities of three species, Agyrodendron actinophyllum, Cardwellia sublimis, and Flindersia brayleyana, commonly found in north Queensland's rainforests were investigated in this study. These particular species are highly valued for their cabinet timber qualities and are being reared in nurseries for use in reforestation trials and programs in north Queensland. Seedlings were initially raised in greenhouse conditions under two layers of shade cloth (16% of full sunlight) and then transferred into full sunlight at three different ages (3 weeks, 10 weeks, 14 weeks). Upon transfer, organ ratios and the direction of dry matter distribution was determined for each species and age group. Approximately 3 months after the seedlings were transferred, the acclimation ability of each species and age group was then determined. Dry matter distribution was found to change with age, irrespective of light environment. Individuals within a species with larger root systems and thicker or more dense leaves had a greater acclimation ability than those with smaller root systems and thinner or less dense leaves. Furthermore, individuals within a species whose dry matter distribution upon transfer was directed towards developing a large root system, and a small photosynthetic area and mass, had a greater acclimation ability than those whose dry matter distribution was directed away from such morphologies. Awareness of these relationships allows a better understanding of seedling response to gap formation in natural forests, and also allows plant nursery operators to make a more informed decision about when to move seedlings to environments with a higher light intensity. 相似文献
In this study,the seeds of wild Petunia Juss.were used as explants to investigate the optimal condition for tissue culture.Several different kinds and concentrations of growth regulators were adopted to produce more multiple bud clumps,callus or roots in this study.The experiments may provide experimental foundation for the rapid propagation technology and establishment of tissue culture system for wild Petunia Juss. 相似文献
Enteral nutrition coupled with bacterial colonization has been shown to have major functional and developmental effects during the postnatal period of neonates. In this study, we developed a highly sensitive premature pig model to elucidate the specific developmental impact of initial bacterial colonization on premature neonates by comparing germ-free and conventionally reared pigs. Thirty-eight preterm pigs (93% gestation) were delivered via caesarean section and reared in either germ-free or conventional isolators for 40–48 h. Pigs were fed either infant milk formula or sow's colostrum. Enteral feeding for two days had trophic effects on gastrointestinal weights, particularly for pancreas, stomach and small intestine (SI). The absence of bacteria in formula fed pigs resulted in a mucosa that appeared more robust and had higher weights for both SI and pancreas, compared to conventional formula fed pigs (P < 0.05). Colostrum fed pigs also had markedly increased mucosal SI proportions, lung and spleen weights compared to conventional formula fed pigs. Colostrum fed pigs and germ-free pigs were similar although stomach, pancreas and distal SI weights were highest for germ-free pigs. The results demonstrate that the initial bacterial colonization interacts with diet to modulate the early neonatal organ development, particularly of the GIT. 相似文献
The objective was to determine the critical N dilution curve of linseed, which is the minimal total N concentration in shoots necessary to produce the maximal shoot dry matter, and to explain possible differences with other C3 species. One main experiment was carried out in 1998/1999 on winter linseed with four levels of fertilizer N. Two plant densities were also studied, the recommended one (600 seeds m−2) and the minimum for canopy closure (150 seeds m−2), in order to investigate the stability with plant density of the critical N dilution curve. Shoot dry weights (WS) and shoot N contents expressed in percentage (NS) were measured for the determination of the critical dilution curve, along with organ N percentages and relative weights. The results of four other experiments were used to validate the critical N dilution curve. Three of these four trials were conducted on winter linseed (one in 1996/1997 and two in 1997/1998) with five levels of fertilizer N, and one on spring linseed in 1999 with six levels of fertilizer N.
The critical N dilution curve of linseed was different from those of other C3 species. The curve was steeper, indicating a greater decrease in the critical shoot N concentration (NSC) as the critical shoot dry weight (WSC) increased. This linseed curve determined with the data of the main experiment was relevant when compared to the data of the four other experiments. Organ weight ratios and N concentration of organs were investigated in a fertilizer N treatment resulting in NS close to the critical N values, NSC. In this treatment, the decrease in NS was the result of both a decrease in the N percentage of all organs and a decrease in the leaf weight ratio. The difference between linseed and other C3 species was mainly due to an acceleration of the dilution of N when leaf emission stopped and the flower bud emission began. At this stage of development, the leaf weight ratio of linseed was less than that of wheat, resulting in lower NS. For a given WS, no significant differences in NS, organ N percentages nor organ weight ratios were observed between the two plant densities. This indicates that the difference between linseed and other C3 species could not result from very high plant densities in linseed. Hence, it is concluded that the linseed N accumulation in shoot is different from other C3 species. 相似文献