Optimum duration of performance tests for evaluating growing pigs for growth and feed efficiency traits

The optimum duration of test for the measurement of ADG, ADFI, feed:gain ratio [which is the reciprocal of the efficiency of gain (G:F) and therefore increases as the efficiency of gain decrease and vice versa], and residual feed intake was examined in growing pigs. Data from 144 hybrid (mainly Large White x Landrace) pigs involved in a longitudinal (n = 54) and serial slaughter (n = 90) experiment were used. The pigs were housed in individual pens from 70 +/- 1 d of age (mean +/- SD) and fed ad libitum a pelleted commercial diet. Feed intake and BW data on pigs that had a minimum of 10-wk records were partitioned into a 14-d adjustment and a 56-d test period. Phenotypic correlations among weekly measurements were used to examine the repeatability of each trait. Changes in phenotypic residual variance and correlation using shortened (7-, 14-, 21-, 28-, 35-, 42-, and 49-d) tests compared with the full-length 56-d test were used as criteria to assess the optimum test duration. The results of the phenotypic correlations among weekly measurements indicated that ADFI, which was characterized by moderate to high correlations (0.41 to 0.81), was more repeatable than ADG, which was characterized by low correlations (0.00 to 0.43). Mean gut fill (n = 107) was 4.2% of BW but was characterized by large variation among the pigs (SD = 1.8; CV = 42.2%). This variation in gut fill was a major contributor to the low repeatability of the measurement of ADG. These repeatability results indicated that ADG, rather than ADFI, will determine the optimum duration of test for the feed efficiency traits. The results of the shortened relative to the full-length test indicate that for growing pigs under good nutrition and ad libitum feeding, a 28-d test was adequate for the measurement of feed intake, whereas a 35-d test was required to measure ADG, feed:gain ratio, and residual feed intake without compromising the accuracy of measurement.     

Periodic vicissitudes of different concentrations of a developed prototype killed S. aureus mastitis vaccine on immune modulators,mediators and immunoglobulins in cows

Tropical Animal Health and Production - Mastitis is the inflammation of the mammary gland due to microbial infiltration causing a reduced mammary function. This study aims at developing a vaccine...     

Biotic, molecular, and phylogenetic characterization of bean calico mosaic virus, a distinct begomovirus species with affiliation in the squash leaf curl virus cluster

ABSTRACT Bean calico mosaic virus (BCMoV), a whitefly-transmitted geminivirus from Sonora, Mexico, was purified, and the genome components were cloned and sequenced. Purified viral fractions and cloned genome components were infectious by biolistic inoculation to bean, completing Koch's postulates for both. The B biotype of the whitefly Bemisia tabaci efficiently transmitted both native virus and progeny virus derived from cloned DNA inoculum. Host ranges of native virus and of progeny virus derived from cloned DNA were identical based upon whitefly and biolistic mediated transmission, respectively. BCMoV has a relatively wide experimental host range among begomoviruses known to infect bean, encompassing genera and species within the Fabaceae, Malvaceae, and Solanaceae. BCMoV has a bipartite genome, as do other New World begomoviruses. BCMoV DNA-A shared highest nucleotide sequence identities with squash leaf curl virus-E strain (SLCV-E) and cabbage leaf curl virus (CaLCV) at 80.1 and 80.7%, respectively. BCMoV DNA-B shared highest nucleotide sequence identity with SLCV-E at 70.7%. The common region (CR) sequences of BCMoV and SLCV-E are 73 to 76% identical; however, modular cis-acting elements within the CR involved in replication origin function and recognition are 100% conserved. Phy-logenetic analysis indicated that BCMoV DNA-A shares a most recent common ancestor with the DNA-A of two viruses that also occur in the Sonoran Desert, SLCV-E and Texas pepper virus (TPV-TAM), and CaLCV from Florida. In contrast, a phylogenetic analysis indicated that BCMoV DNA-B shares a most recent common ancestor with SLCV-E; whereas DNA-B of CaLCV clustered in a separate clade with pepper hausteco virus. Collectively, biological and molecular characteristics indicate that BCMoV is a distinct begomovirus species with the northernmost distribution of any begomovirus isolated from bean in the Americas. Furthermore, the phylogenetic relationships of begomovirus cognate components are not necessarily identical, suggesting that DNA-A and DNA-B of some begomoviruses may have different evolutionary histories.     

Detection of Campylobacter jejuni strains in the water lines of a commercial broiler house and their relationship to the strains that colonized the chickens

Campylobacter jejuni is frequently present in the intestinal tract of commercial broiler chickens, and their drinking water has been proposed to be an initial source of bacteria for newly hatched chicks. We studied three sequential commercial broiler flocks raised in a house from which we had cultured C. jejuni from the nipple waters prior to placement of the first flock. Campylobacter cells were detected by immunofluorescence in the biofilm of the drinking nipples during the weeks when the flock was colonized with C. jejuni but not during weeks when the birds were negative. Campylobacter jejuni was isolated from the drinking water during the growth of the first flock and was present in the birds from all three flocks. Randomly amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) typing with primer OPA11 indicated that seven distinct strains were present within the broiler house. One strain found in drinking water was similar to a strain found in birds in the second flock; however, RAPD-PCR with primer HLW85 showed that the strains were not identical. These results suggest that although the watering system is a potential source of C. jejuni in broiler flocks, the waterborne strain in this study was not detected in the birds.     

Emergence of a New Cucurbit-Infecting Begomovirus Species Capable of Forming Viable Reassortants with Related Viruses in the Squash leaf curl virus Cluster

ABSTRACT Cucurbit leaf curl virus (CuLCV), a whitefly-transmitted geminivirus previously partially characterized from the southwestern United States and northern Mexico, was identified as a distinct bipartite begomovirus species. This virus has near sequence identity with the previously partially characterized Cucurbit leaf crumple virus from California. Experimental and natural host range studies indicated that CuLCV has a relatively broad host range within the family Cucurbitaceae and also infects bean and tobacco. The genome of an Arizona isolate, designated CuLCV-AZ, was cloned and completely sequenced. Cloned CuLCV-AZ DNA A and B components were infectious by biolistic inoculation to pumpkin and progeny virus was transmissible by the whitefly vector, Bemisia tabaci, thereby completing Koch's postulates. CuLCV-AZ DNA A shared highest nucleotide sequence identity with Squash leaf curl virus-R (SLCV-R), SLCV-E, and Bean calico mosaic virus (BCaMV) at 84, 83, and 80%, respectively. The CuLCV DNA B component shared highest nucleotide sequence identity with BCaMV, SLCV-R, and SLCV-E at 71, 70, and 68%, respectively. The cis-acting begomovirus replication specificity element, GGTGTCCTGGTG, in the CuLCV-AZ origin of replication is identical to that of SLCV-R, SLCV-E, and BCaMV, suggesting that reassortants among components of CuLCV-AZ and these begomoviruses may be possible. Reassortment experiments in pumpkin demonstrated that both reassortants of CuLCV-AZ and SLCV-E A and B components were viable. However, for CuLCV-AZ and SLCV-R, only one reassortant (SLCV-R DNA A/CuLCV-AZ DNA B) was viable on pumpkin, even though the cognate component pairs of both viruses infect pumpkin. These results demonstrate that reassortment among sympatric begomovirus species infecting cucurbits are possible, and that, if generated in nature, could result in begomoviruses bearing distinct biological properties.     

Chemical Control of Weeds in Cotton in the Sudan Gezira

Abstract

Early work on chemical weed control in the fallow towards the end of the Second World War is reviewed briefly. Attempts to use contact residual herbicides as pre-emergence applications in the early 1950s are also described.

Pre-sowing applications of residual herbicides were found more phytotoxic to cotton than pre-emergence applications. The closer the time of pre-sowing application to cotton sowing, the more effective was suppression of weeds competing with cotton.

Mechanical cultivations and particularly disc ploughing and rotovation to a depth of more than 15 cm were found more effective in the control of Cynodon dactylon than pre-sowing herbicide applications.

Fluometuron, noruron and prometryne were found very effective as pre-emergence herbicides. Optimum rates of application were found to be only 25-50% of rates recommended for the USA and other countries. Resistant weeds included Cyperus rotundus, Cynodon dactylon, and Rhyncosia memnonia.

A large-scale trial with fluometuron, covering 125 feddans at Wad el Nairn showed that yields obtained were equal to those with normal weeding. Costs of herbicide treatments were also comparable to costs of hand-weeding in a year of exceptionally low hand-weeding costs.     

Clinical and subclinical colloid goitre in adult camels (Camelus dromedarius) at Kordofan region of Sudan

Colloid goitre was diagnosed in adult camels in the Kordofan region of the Sudan. The disease is characterized by gross enlargement of the thyroid, histopathological follicular changes, reproductive disorders, low concentrations of circulating thyroid hormones (T3, T4) and normocytic normochromic anaemia. The possible cause of the condition is discussed and iodine supplementation is recommended.