来源于新疆的3 个苹果褪绿叶斑病毒分离物的分子变异研究

Apple chlorotic leaf spot virus (ACLSV) isolates from Korla pear (KI-2), New pear no. 7 (XI-1) and Red Fuji apple (API-4) were collected from XinJiang and characterized by analyzing sequences of their near genomic 3忆-terminal. The RT-PCR products were cloned, and analyzed by single-strand conforma-tion polymorphism (SSCP). Eight out of 39 collected positive clones showing different SSCP patterns were sequenced. The results showed that the amplified products had sizes ranging 676 - 703 bp, including partial coat protein (CP) gene (506 bp, accounts for 87% of the complete cp gene) and 3忆-terminal non-coding re-gion (3忆NCR) sequences. The cp gene sequences from isolate KI-2 showed a high intra-isolate divergence,with 84. 8% - 85. 4% identities at the nucleotide (nt) level, and the intra-isolate identities were 99. 8 % and 92.5% - 99. 8 % for isolate XI-1 and API-4, respectively. Phylogenetic analysis on the nt sequences of cpgene showed that the analyzed ACLSV variants from three isolates fell into two different clusters. A variant KI-2-6 from KI-2 was clustered into a group with an apple isolate aclsv-c from China and a plum isolated from France, and all other variants fell into a large cluster. The 3忆NCR sequences of these variants were identical ranging 80. 6% - 100 % .     

杂交棉标杂A1磷素营养特性研究

一、材料与方法 1.供试材料及处理 试验安排在农6师103团1连。土壤质地为壤土,碱解氮70.96毫克/千克,速效磷8.89毫克/千克,速效钾68.65毫克/千克,供试棉花品种为标杂A1,供试肥料,氮肥为尿素,磷肥为三料磷,钾肥为硫酸钾。试验采用＂3414＂方案设计。氮667米2常规施肥量为35千克,磷667米2常规施肥量为12.5千克,钾667米2常规施肥量9千克,小区面积40.46米2,磷、     

功在当代 利惠千秋──贵州省雷山县以工代赈坡改梯调查

功在当代利惠千秋──贵州省雷山县以工代赈坡改梯调查贵州韦向东贵州省雷山县９１、乡镇１５６个村散落在苗岭主峰──雷公山四周，境内山高坡陡，阴冷烂锈田占耕地面积中的比重大，粮食产量低，人均耕地面积和人均占有粮食少，解决全县１３万人民的温饱问题一直是摆在父...