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Objective To evaluate the antituberculous activities of Huangkuisu (HISS). Meth-ods lOStandard human strains of mycobacterium tuberculosis (H37RV) and multi-drug resistant TBstrainswere inoculated in different concentrations of HISS, blank control and positive TB drug culture medium[()observe the growth of mycobacterium tuberculosis and evaluate the antibacterial activity in vitro.②Certain number of standard strains of Mycobacterium tuberculosis H37Rv and drug-resistant strains were injected into the tail vein[()establish a mouse model of mycobacterium tuberculosis infection. The mice were randomly divided into treatment group, rifampicin, HISS treatment group treated lasting for 6 weeks; the control group was given normal saline. The antibacterial activity in vivo were evaluated[()compare the treatment results according[()the pathological changes in lung and spleen tissues (HE staining and acid-fast staining) and charging amount of bacteria. Results lOthe bactreial colonies were grew normally in model group, but were not grew in 相似文献
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剪股颖粒线虫[Anguina agrostis (Steinbuch,1799) Filipjev,1936]、小麦粒线虫[Anguina tritici (Steinbuch,1799) Filipjev,1936]和维氏粒线虫[Anguina wevelli (Van den Berg,1985) Siddiqi,2000]都是重要的植物病原线虫,它们在成熟种子和虫瘿中的虫态通常是幼虫,而这3种线虫的幼虫形态非常相似,难以根据其特征对它们进行快速准确的种类鉴定。本研究根据这3种线虫的rDNA-ITS区域序列,分别设计筛选了特异性引物AgrF1/AgrR1、TriF1/TriR1、WevF1/WevR1,构建了这3种线虫单条幼虫多重PCR检测体系,获得3个大小差异明显的片段,表明这些引物设计合理,适合这3种线虫的快速准确检测。 相似文献
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本文对国内外研究者设计的11对PCR引物检测柑桔黄龙病菌的特异性进行了比较。其中包括5对一步法PCR引物(A2/J5、GB1/GB3、MP1/MP2、CQULA03F/CQULA03R和CLA-F2/CLA-R2)和6对巢式PCR引物(P1/P2、P3/P4、LP1/LP2、LP3/LP4、DP1/DP2和DP3/DP4)。结果发现,MP1/MP2和CLA-F2/CLA-R2 2对引物具有非特异性,不能区分亚洲柑桔黄龙病菌和非洲柑桔黄龙病菌,其余的9对引物特异性较好。本文旨在为研究者选择柑桔黄龙病菌扩增引物提供参考。 相似文献
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根结线虫属(Meloidogyne spp.)是专性内寄生物,寄主范围广,全球分布,给世界农业生产造成严重为害。生殖方式主要有孤雌生殖和两性融合。系统发育学研究指出,种间杂交可能在根结线虫多样化的进程中起着重要作用。越来越多的证据表明,基因组范围内的序列重复和水平基因转移为根结线虫的多样性提供了遗传基础。南方根结线虫(Meloidogyne incognita)和北方根结线虫(Meloidogyne hapla)基因组测序的完成和注释也为研究根结线虫的多样性提供了重要线索。根结线虫的多样性给鉴定和防治工作带来了巨大挑战,需要寻找更加有效的方法。 相似文献