Aerobic bacterial flora of the nasal cavity in Gulf of California sea lion (Zalophus californianus) pups

Nasal swab samples from clinically healthy California sea lions pups (Zalophus californianus) from six different reproductive rookeries in the Gulf of California were collected to determine the type and frequency of the representative aerobic bacterial microflora of their nasal mucosa. A total of 114 samples were examined and 100 bacterial isolates were identified and typified by microbiological and biochemical standard tests. Fifty four isolates corresponded to Gram positive bacteria (54%) and 46 isolates to Gram negative bacteria (46%). Fifteen bacterial genera were identified, including Micrococcus, Arcanobacterium, Corynebacterium, Moraxella, Neisseria, Escherichia, Kurthia, Acinetobacter, Staphylococcus, Brevibacillus, Bacillus, Klebsiella, Stenotrophomonas, Pseudomonas and Aeromonas. The most frequently isolated genera were Moraxella (24%), Micrococcus (18%), and Corynebacterium (15%). These results show the presence in the nasal cavity of sea lions of several microorganisms. Although considered part of the normal microflora, they may also be opportunistic pathogens for their hosts and may act as a potential natural sentinel of environmental changes.     

Outbreak of cryptosporidiosis among veterinary students

In January 2007, six veterinary students became infected with Cryptosporidium species, and records indicated that another student had been diagnosed in November 2006. It was established that the seven students had worked with cattle from the same farm. Microbiological tests indicated that they were infected with Cryptosporidium parvum. Subtyping by sequence analysis indicated a common source of infection for five of the students, but there was insufficient material to type the other two samples. Investigations indicated that the outbreak was caused by a lapse in hygiene, particularly handwashing, on a farm with enzootic C parvum in calves.     

Obtaining and characterizing Pleurotus ostreatus strains for commercial cultivation under warm environmental conditions

Pleurotus ostreatus strains obtained by interbreeding were cultivated under warm conditions (27 °C), with the aim to identify germoplasm adequate to commercial production. Based on the radial growth rate of monokaryotic mycelia isolated from two P. ostreatus strains, a culture isolate was selected from each type of compatibility, and interbred to obtain 16 strains. Pasteurized barley straw was used as the substrate for mushroom production. The mean biological efficiency of the strains was 84.17% and the production rate was 1.76%. In vitro laccase activity was tested for both parental strains and the four most productive strains of progeny. The mean enzyme activity of the strains was 1.21 μg mol/disc/ABTS oxidized. These results show the high potential of these strains for their use in substrate delignification and the production of basidiocarps under natural environmental conditions of warm regions.     

Nutritional reference values for Opuntia ficus‐indica determined by means of the boundary‐line approach

Optimum concentrations and/or sufficiency ranges of nutrients are useful for a correct diagnosis and improvement of nutrient status of cultivated plants. To develop boundary‐line approach (BLA) standards for Opuntia ficus‐indica L., a database of N, P, K, Ca, and Mg concentrations in 1‐year‐old cladodes and cladodes fresh‐matter yield was used. The BLA optimum concentrations (associated with estimated maximum yield) for O. ficus‐indica were: N = 13.1 g kg^–1, P = 3.2 g kg^–1, K = 44.4 g kg^–1, Ca = 38.1 g kg^–1, and Mg = 17.3 g kg^–1. The BLA sufficiency ranges at 95% yield were 8.4–20.3 g kg^–1 for N, 2.4–4.2 g kg^–1 for P, 38.2–50.8 g kg^–1 K, 31.8–45.2 g kg^–1 for Ca, and 14.3–20.9 g kg^–1 for Mg. The BLA standards are comparable to those obtained in a previous study using compositional‐nutrient‐diagnosis (CND) approach.     

Steps toward sustainable ranching: An emergy evaluation of conventional and holistic management in Chiapas, Mexico

Conventional ranching in Chiapas, Mexico typically includes annual pasture burns and agrochemical use that decrease the biodiversity and forest cover of ranch lands. Members of a holistic ranching “club” in the Frailesca region of Chiapas, Mexico have moved away from this conventional management by eliminating burns and agrochemicals from their systems after decades of use because they believed that the land and their production process were growing unhealthy; they were further motivated by extension courses on holistic ranching. They have also implemented sophisticated systems of rotational grazing and diversified the use of trees. For this study all seven holistic ranchers and 18 neighboring conventional ranchers were interviewed about their cattle ranches and production strategies. An emergy analysis was conducted to compare the resource use, productivity and sustainability of the conventional and holistic ranches. Holistic ranches were found to have double the emergy sustainability index (ESI) values of conventional ranches, and the emergy yield ratio was 25% higher in holistic systems. Government assistance programs were found to have a negative impact on the ESI and were variably administered among holistic ranchers during the year of emergy evaluation. Overall improved emergy sustainability did not decrease milk nor cattle productivity. Transformities and specific emergies, the emergy of one type required to make a unit of energy (transformity) or mass (specific emergy) of another type, did not differ between conventional and holistic systems. Transformities for milk production ranged between 3.4E5 and 1.2E7 solar emjoules/joule (sej/J). Specific emergy for cattle production ranged from 3.5E10 to 1.5E11 sej/g. To improve the ESI assistance programs could be re-targeted toward incentive programs for increased forest cover in ranching systems and startup costs for holistic ranching. The results from this study show that productivity can be maintained as the sustainability of rural dairy ranches is increased. These results also show that local knowledge and understanding of the surrounding ecosystem can drive positive environmental change in production systems.     

Technical inefficiency of Vietnamese pangasius farming: A data envelopment analysis

Vietnamese pangasius farming needs to produce efficiently to compete in world markets. This study investigates the input- and output-specific technical inefficiency of Vietnamese pangasius farmers. First, we used a Russell-type (input–output) directional distance function to estimate the input- and output-specific technical inefficiency. Second, we applied a bootstrap truncated regression to analyze the factors influencing these technical inefficiencies. Results show that the main challenges for enhancing the performance of Vietnamese pangasius production are inadequate use of capital assets (inefficiency of 42%) and improper methods to achieve higher fish yield (inefficiency of 30%). Input-specific technical inefficiency (pond area and feed) is negatively associated with the experience and education level of pangasius farmers. Location of the farm in a saltwater intrusion area is positively associated with the inefficiency of producing fish. Outcomes of this study are useful to identify successful strategies to minimize the use of inputs while simultaneously maximizing fish production.     

Isolation of <Emphasis Type="Italic">Brucella melitensis</Emphasis> from a RB51-vaccinated seronegative goat

The purpose of this study is to determine the etiology of abortions presented in a goat herd declared as free of brucellosis and vaccinated with RB51 located in Mexico. The serological diagnosis of brucellosis in 33 animals was performed. The study included three goats that aborted in the last third of gestation and 15 goats that gave birth normally; samples of milk and vaginal exudate were subjected to bacteriological study. All animals were negative for serological diagnosis, and isolation of Brucella melitensis was achieved in a single goat from vaginal exudate. However, the particularity is that this goat was negative to the card, indirect ELISA, and radial immunodiffusion tests. Isolation of a field strain was confirmed by biochemical test resistance to rifampicin and PCR. It is concluded that a goat which aborted in the last third of gestation was found spreading B. melitensis through vaginal discharge despite being vaccinated with RB51 and seronegative for brucellosis.     

Involving local people in the conservation of the critically endangered freshwater turtle Dermatemys mawii in southern Mexico

1. The Central American river turtle Dermatemys mawii is one of the most endangered species in the world, and continues to decline. In Mexico, this turtle is legally protected but, in the rural areas where it is found, local people are not aware of why it is important to conserve it.
2. This study confirmed that D. mawii inhabits lakes in southern Mexico, and that these areas are among the last refuges for this species inside and outside protected areas in the wild.
3. A lack of knowledge about its endangered status among local people means that a strategy of environmental education is needed.

     

Effects of body condition and supplementation on ovarian function,growth factors and response to estrus synchronization in postpartum beef cows in Tamaulipas,Mexico

ABSTRACT

Eighteen beef cows with their calves were assigned to 3 groups considering body conditions scores at calving (BCSC): T1 (high BCSC, maintained throughout the study); T2 (low BCSC, increased during the study); and T3 (low BCSC, maintained throughout). An estrus synchronization protocol was implemented, with fixed time artificial insemination on day 70. Follicular or luteal growth, and pregnancy rate were similar between groups. Of all cows, five showed multiple ovulations (2–6); increasing number of ovulations reduced preovulatory follicle diameter and individual luteal volume (P?<?0.05), but total luteal volume was greater in cows with 2 or 3 ovulations on day 14 after insemination (P?<?0.05). Estradiol and progesterone levels were associated to follicular and luteal growth, respectively. No difference was found in IGF1 or GDF9 levels between groups or associated to fertility. No association was found between BCS, IGF1 or GDF9 levels and the reproductive process.     

Semi-Intensive Commercial Grow-Out of Penaeus vannamei Fed Diets Containing Differing Levels of Crude Protein During Wet and Dry Seasons in Honduras

Shrimp were grown under ideal management conditions during two distinct seasons of the year at stocking densities used most often in Honduras with the objective of evaluating the usefulness of high protein diets. A randomized design in 2 × 2 factorial arrangement was used to test a diet composed of either 20 or 40% crude protein in earthen ponds that were stocked with juvenile Penaeus vannamei at 5 to 11/m². The study was repeated during wet and dry seasons. Dietary protein level had no significant effect ( P > O .05) on survival, yield, or average weight of shrimp at either density during either season. Higher stocking rates yielded significantly greater shrimp production during both seasons. Mean shrimp weight in high density ponds was significantly lower than mean shrimp weight in low density ponds during the wet season, but there was no significant weight difference because of stocking density during the dry season. Mean survival was significantly lower at the higher stocking rate during the dry season. Net income was negative during the dry season, particularly at the high stocking density. Mean production was 240% greater in the wet season than in the dry season. Diets offered P. vannamei stocked at 5 to 11/m² should contain no more than 20% protein, regardless of season. Higher dietary protein levels increase costs and waste nitrogen without resulting in greater shrimp yields. The high stocking density might increase profitability in the wet season, but long term sustainable production may be more feasible at lower stocking rates because of reduced nutrient wastes.